The ADE2 gene from Saccharomyces cerevisiae: sequence and new vectors - PubMed (original) (raw)
The ADE2 gene from Saccharomyces cerevisiae: sequence and new vectors
A Stotz et al. Gene. 1990.
Abstract
We have determined the sequence of a DNA fragment encoding the ADE2 gene from Saccharomyces cerevisiae. A DNA fragment of 2241 bp capable of complementing ade2 mutations was modified so it is available as a single BglII fragment for use in yeast vectors or for gene disruptions. The minimal fragment codes for a putative protein which is highly similar to the protein encoded by the ADE6 gene from Schizosaccharomyces pombe and to the proteins encoded by the purEK operon of Escherichia coli.
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