Expression of the structural proteins of dengue 2 virus and yellow fever virus by recombinant vaccinia viruses - PubMed (original) (raw)

Expression of the structural proteins of dengue 2 virus and yellow fever virus by recombinant vaccinia viruses

Y S Hahn et al. Arch Virol. 1990.

Abstract

Vaccinia virus recombinants were constructed which contained cDNA sequences encoding the structural region of dengue 2 virus (PR159/S1 strain) or yellow fever virus (17D strain). The flavivirus cDNA sequences were expressed under the control of the vaccinia 7.5k early/late promotor. Cultured cells infected with these recombinants expressed immunologically reactive flavivirus structural proteins, precursor prM and E. These proteins appeared to be cleaved and glycosylated properly since they comigrated with the authentic proteins from dengue 2 virus- and yellow fever virus-infected cells. Mice immunized with the dengue/vaccinia recombinant showed a dengue-specific immune response that included low levels of neutralizing antibodies. Immunization of mice with the yellow fever/vaccinia recombinant was less effective at inducing an immune response to yellow fever virus and in only some of the mice were low titers of neutralizing antibodies produced.

PubMed Disclaimer

Similar articles

• Yellow fever virus/dengue-2 virus and yellow fever virus/dengue-4 virus chimeras: biological characterization, immunogenicity, and protection against dengue encephalitis in the mouse model.
  Chambers TJ, Liang Y, Droll DA, Schlesinger JJ, Davidson AD, Wright PJ, Jiang X. Chambers TJ, et al. J Virol. 2003 Mar;77(6):3655-68. doi: 10.1128/jvi.77.6.3655-3668.2003. J Virol. 2003. PMID: 12610141 Free PMC article.
• Recombinant chimeric yellow fever-dengue type 2 virus is immunogenic and protective in nonhuman primates.
  Guirakhoo F, Weltzin R, Chambers TJ, Zhang ZX, Soike K, Ratterree M, Arroyo J, Georgakopoulos K, Catalan J, Monath TP. Guirakhoo F, et al. J Virol. 2000 Jun;74(12):5477-85. doi: 10.1128/jvi.74.12.5477-5485.2000. J Virol. 2000. PMID: 10823852 Free PMC article.
• Recombinant vaccinia virus producing the prM and E proteins of yellow fever virus protects mice from lethal yellow fever encephalitis.
  Pincus S, Mason PW, Konishi E, Fonseca BA, Shope RE, Rice CM, Paoletti E. Pincus S, et al. Virology. 1992 Mar;187(1):290-7. doi: 10.1016/0042-6822(92)90317-i. Virology. 1992. PMID: 1736531
• Recombinant vaccinia viruses co-expressing dengue-1 glycoproteins prM and E induce neutralizing antibodies in mice.
  Fonseca BA, Pincus S, Shope RE, Paoletti E, Mason PW. Fonseca BA, et al. Vaccine. 1994;12(3):279-85. doi: 10.1016/0264-410x(94)90206-2. Vaccine. 1994. PMID: 8165861
• Attenuation and immunogenicity of recombinant yellow fever 17D-dengue type 2 virus for rhesus monkeys.
  Galler R, Marchevsky RS, Caride E, Almeida LF, Yamamura AM, Jabor AV, Motta MC, Bonaldo MC, Coutinho ES, Freire MS. Galler R, et al. Braz J Med Biol Res. 2005 Dec;38(12):1835-46. doi: 10.1590/s0100-879x2005001200012. Epub 2005 Nov 9. Braz J Med Biol Res. 2005. PMID: 16302098

Cited by

• Flavivirus premembrane protein cleavage and spike heterodimer secretion require the function of the viral proteinase NS3.
  Lobigs M. Lobigs M. Proc Natl Acad Sci U S A. 1993 Jul 1;90(13):6218-22. doi: 10.1073/pnas.90.13.6218. Proc Natl Acad Sci U S A. 1993. PMID: 8392191 Free PMC article.
• NS2B-3 proteinase-mediated processing in the yellow fever virus structural region: in vitro and in vivo studies.
  Amberg SM, Nestorowicz A, McCourt DW, Rice CM. Amberg SM, et al. J Virol. 1994 Jun;68(6):3794-802. doi: 10.1128/JVI.68.6.3794-3802.1994. J Virol. 1994. PMID: 8189517 Free PMC article.
• Insect-transmitted vertebrate viruses: flaviviridae.
  Ludwig GV, Iacono-Connors LC. Ludwig GV, et al. In Vitro Cell Dev Biol Anim. 1993 Apr;29A(4):296-309. doi: 10.1007/BF02633958. In Vitro Cell Dev Biol Anim. 1993. PMID: 8100566 Free PMC article. Review.
• Protective efficacy in mice of a secreted form of recombinant dengue-2 virus envelope protein produced in baculovirus infected insect cells.
  Delenda C, Frenkiel MP, Deubel V. Delenda C, et al. Arch Virol. 1994;139(1-2):197-207. doi: 10.1007/BF01309465. Arch Virol. 1994. PMID: 7826210
• Processing of dengue virus type 2 structural proteins containing deletions in hydrophobic domains.
  Gruenberg A, Wright PJ. Gruenberg A, et al. Arch Virol. 1992;122(1-2):77-94. doi: 10.1007/BF01321119. Arch Virol. 1992. PMID: 1729986

References

1. 1. J Virol. 1985 Oct;56(1):227-39 - PubMed
2. 1. Nature. 1970 Aug 15;227(5259):680-5 - PubMed
3. 1. J Virol. 1979 Feb;29(2):705-15 - PubMed
4. 1. Virology. 1989 Apr;169(2):365-76 - PubMed
5. 1. J Virol. 1989 May;63(5):1852-60 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources

• Other Literature Sources

  • The Lens - Patent Citations Database