Thiamin biosynthesis: still yielding fascinating biological chemistry - PubMed (original) (raw)

Review

Thiamin biosynthesis: still yielding fascinating biological chemistry

Tadhg P Begley et al. Biochem Soc Trans. 2012.

Abstract

The present paper describes the biosynthesis of the thiamin thiazole in Bacillus subtilis and Saccharomyces cerevisiae. The two pathways are quite different: in B. subtilis, the thiazole is formed by an oxidative condensation of glycine, deoxy-D-xylulose 5-phosphate and a protein thiocarboxylate, whereas, in S. cerevisiae, the thiazole is assembled from glycine, NAD and Cys205 of the thiazole synthase.

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Figures

Figure 1

The bacterial thiamin biosynthetic pathway.

Figure 2

Four additional examples of protein-thiocarboxylate-dependent biosynthetic pathways. A) Molybdopterin biosynthesis in bacteria, B) Cysteine biosynthesis in Mycobacterium tuberculosis, C) Homocysteine biosynthesis in Wolinella succinogenes, D) Thioquinolobactin biosynthesis in Pseudomonas fluorescens.

Figure 3

Mechanistic proposal for the formation of the thiazole tautomer 7.

Figure 4

Thiamin pyrophosphate biosynthesis in S. cerevisiae.

Figure 5

Mechanistic proposal for the THI4 mediated formation of ADP-thiazole 64.

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References

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Thiamin biosynthesis: still yielding fascinating biological chemistry - PubMed (original) (raw)