K-252a inhibits the response of tomato cells to fungal elicitors in vivo and their microsomal protein kinase in vitro - PubMed (original) (raw)
K-252a inhibits the response of tomato cells to fungal elicitors in vivo and their microsomal protein kinase in vitro
D G Grosskopf et al. FEBS Lett. 1990.
Free article
Abstract
Two characteristic responses of plant cells to fungal elicitors, induction of phenylalanine ammonia-lyase activity and of ethylene biosynthesis, were studied in suspension-cultured tomato cells. Induction of both responses was completely blocked by 500 nM K-252a, a known inhibitor of mammalian protein kinases. About 100 nM K-252a caused half-maximal inhibition. In vitro, K-252a inhibited protein kinase activity in microsomal preparations from tomato cells. Inhibition was competitive with respect to ATP and had a Ki of about 15 nM. Thus, protein kinases sensitive to K-252a occur in plants and might be important for the plant's response to fungal elicitors.
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