Extracellular-matrix tethering regulates stem-cell fate - PubMed (original) (raw)

. 2012 May 27;11(7):642-9.

doi: 10.1038/nmat3339.

Julien E Gautrot, John T Connelly, Daniel G T Strange, Yuan Li, Michelle L Oyen, Martien A Cohen Stuart, Heike Boehm, Bojun Li, Viola Vogel, Joachim P Spatz, Fiona M Watt, Wilhelm T S Huck

Affiliations

• PMID: 22635042
• DOI: 10.1038/nmat3339

Extracellular-matrix tethering regulates stem-cell fate

Britta Trappmann et al. Nat Mater. 2012.

Erratum in

• Nat Mater. 2012 Aug;11(8):742

Abstract

To investigate how substrate properties influence stem-cell fate, we cultured single human epidermal stem cells on polydimethylsiloxane (PDMS) and polyacrylamide (PAAm) hydrogel surfaces, 0.1 kPa-2.3 MPa in stiffness, with a covalently attached collagen coating. Cell spreading and differentiation were unaffected by polydimethylsiloxane stiffness. However, cells on polyacrylamide of low elastic modulus (0.5 kPa) could not form stable focal adhesions and differentiated as a result of decreased activation of the extracellular-signal-related kinase (ERK)/mitogen-activated protein kinase (MAPK) signalling pathway. The differentiation of human mesenchymal stem cells was also unaffected by PDMS stiffness but regulated by the elastic modulus of PAAm. Dextran penetration measurements indicated that polyacrylamide substrates of low elastic modulus were more porous than stiff substrates, suggesting that the collagen anchoring points would be further apart. We then changed collagen crosslink concentration and used hydrogel-nanoparticle substrates to vary anchoring distance at constant substrate stiffness. Lower collagen anchoring density resulted in increased differentiation. We conclude that stem cells exert a mechanical force on collagen fibres and gauge the feedback to make cell-fate decisions.

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Comment in

• Stem-cell differentiation: Anchoring cell-fate cues.
  Chaudhuri O, Mooney DJ. Chaudhuri O, et al. Nat Mater. 2012 Jun 21;11(7):568-9. doi: 10.1038/nmat3366. Nat Mater. 2012. PMID: 22717486 No abstract available.

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