Transcription by eukaryotic RNA polymerases A and B of chromatin assembled in vitro - PubMed (original) (raw)

Comparative Study

Transcription by eukaryotic RNA polymerases A and B of chromatin assembled in vitro

B Wasylyk et al. Eur J Biochem. 1979.

Free article

Abstract

Chromatin was assembled in vitro from simian virus 40 DNA form I and the calf-thymus four histones H2A, H2B, H3 and H4. Transcription with calf thymus RNA polymerases A and B (I and II) was greatly inhibited. Nucleosomes were found to inhibit both RNA chain initiation and elongation. The inhibition of elongation could be relieved by increasing ionic strength, suggesting that electrostatic interactions between histone octamer and DNA have to be broken for RNA polymerase to transcribe DNA organised into nucleosomes.

PubMed Disclaimer

Similar articles

• Comparative study of calf thymus and wheat germ RNA polymerase II: stability of initiation complexes and elongation rates.
  Saragosti S, Lescure B, Yaniv M. Saragosti S, et al. Biochem Biophys Res Commun. 1979 Jun 13;88(3):1077-84. doi: 10.1016/0006-291x(79)91518-3. Biochem Biophys Res Commun. 1979. PMID: 223568 No abstract available.
• Studies on the mechanism of transcription of nucleosomal complexes.
  Wasylyk B, Chambon P. Wasylyk B, et al. Eur J Biochem. 1980 Jan;103(2):219-26. doi: 10.1111/j.1432-1033.1980.tb04306.x. Eur J Biochem. 1980. PMID: 6244943
• Activation of chromatin by acetylation of histone side chains.
  Marushige K. Marushige K. Proc Natl Acad Sci U S A. 1976 Nov;73(11):3937-41. doi: 10.1073/pnas.73.11.3937. Proc Natl Acad Sci U S A. 1976. PMID: 1069278 Free PMC article.
• Transcription through the nucleosome.
  Kujirai T, Kurumizaka H. Kujirai T, et al. Curr Opin Struct Biol. 2020 Apr;61:42-49. doi: 10.1016/j.sbi.2019.10.007. Epub 2019 Nov 29. Curr Opin Struct Biol. 2020. PMID: 31790919 Review.
• Histone dynamics during transcription: exchange of H2A/H2B dimers and H3/H4 tetramers during pol II elongation.
  Thiriet C, Hayes JJ. Thiriet C, et al. Results Probl Cell Differ. 2006;41:77-90. doi: 10.1007/400_009. Results Probl Cell Differ. 2006. PMID: 16909891 Review.

Cited by

• Histone 3 lysine 4 monomethylation supports activation of transcription in S. cerevisiae during nutrient stress.
  Deshpande N, Jordan R, Henderson Pozzi M, Bryk M. Deshpande N, et al. Curr Genet. 2022 Apr;68(2):181-194. doi: 10.1007/s00294-022-01226-2. Epub 2022 Jan 18. Curr Genet. 2022. PMID: 35041077 Free PMC article.
• Advances and Limitations of Current Epigenetic Studies Investigating Mammalian Axonal Regeneration.
  Palmisano I, Di Giovanni S. Palmisano I, et al. Neurotherapeutics. 2018 Jul;15(3):529-540. doi: 10.1007/s13311-018-0636-1. Neurotherapeutics. 2018. PMID: 29948919 Free PMC article. Review.
• Independent manipulation of histone H3 modifications in individual nucleosomes reveals the contributions of sister histones to transcription.
  Zhou Z, Liu YT, Ma L, Gong T, Hu YN, Li HT, Cai C, Zhang LL, Wei G, Zhou JQ. Zhou Z, et al. Elife. 2017 Oct 13;6:e30178. doi: 10.7554/eLife.30178. Elife. 2017. PMID: 29027902 Free PMC article.
• Modeling exon expression using histone modifications.
  Zhu S, Wang G, Liu B, Wang Y. Zhu S, et al. PLoS One. 2013 Jun 25;8(6):e67448. doi: 10.1371/journal.pone.0067448. Print 2013. PLoS One. 2013. PMID: 23825663 Free PMC article.
• Chromatin and transcription in yeast.
  Rando OJ, Winston F. Rando OJ, et al. Genetics. 2012 Feb;190(2):351-87. doi: 10.1534/genetics.111.132266. Genetics. 2012. PMID: 22345607 Free PMC article. Review.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Wiley