Isolation of human eosinophils - PubMed (original) (raw)

Isolation of human eosinophils

Praveen Akuthota et al. Curr Protoc Immunol. 2012 Aug.

Abstract

Highly purified eosinophils can be isolated from peripheral blood by negative selection using an antibody-based magnetic negative selection protocol. The basic protocol describes a sequential fractionation of peripheral blood in which CD16+ granulocytes are enriched first from whole blood, followed by isolation of eosinophils. This technique is easy to use, fast, and highly reproducible. Support protocols describe a staining methods that can be used to evaluate the purity of eosinophils and differentiation from other leukocyte populations.

© 2012 by John Wiley & Sons, Inc.

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