SARS-CoV regulates immune function-related gene expression in human monocytic cells - PubMed (original) (raw)

Wanchung Hu et al. Viral Immunol. 2012 Aug.

Abstract

Severe acute respiratory syndrome (SARS) is characterized by acute respiratory distress syndrome (ARDS) and pulmonary fibrosis, and monocytes/macrophages are the key players in the pathogenesis of SARS. In this study, we compared the transcriptional profiles of SARS coronavirus (SARS-CoV)-infected monocytic cells against that infected by coronavirus 229E (CoV-229E). Total RNA was extracted from infected DC-SIGN-transfected monocytes (THP-1-DC-SIGN) at 6 and 24 h after infection, and the gene expression was profiled in oligonucleotide-based microarrays. Analysis of immune-related gene expression profiles showed that at 24 h after SARS-CoV infection: (1) IFN-α/β-inducible and cathepsin/proteasome genes were downregulated; (2) hypoxia/hyperoxia-related genes were upregulated; and (3) TLR/TLR-signaling, cytokine/cytokine receptor-related, chemokine/chemokine receptor-related, lysosome-related, MHC/chaperon-related, and fibrosis-related genes were differentially regulated. These results elucidate that SARS-CoV infection regulates immune-related genes in monocytes/macrophages, which may be important to the pathogenesis of SARS.

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Figures

FIG. 1.

FIG. 1.

Heat map image of microarray analysis result of SARS-CoV- and CoV-229E infected THP-1-DC-SIGN cells (s6M, SARS 6 h mean; s6A, SARS 6 h sample A; s6B, SARS 6 h sample B; e6M, CoV-229E 6 h mean; e6A, Cov-229E 6 h sample A; e6B: CoV-229E 6 h sample B; s24M, SARS 24 h mean; s24A, SARS 24 h sample A; s24B, SARS 24 h sample B; e24M, CoV-229E 24 h mean; e24A, Cov-229E 24 h sample A; e24B, CoV-229E 24 h sample B). Color images available online at

www.liebertpub.com/vim

FIG. 2.

FIG. 2.

Pathway analysis of the gene-to-gene networks of the IL-8 and proteasome families. Red and green colors denote upregulation and downregulation, respectively. Darker color indicates greater up- or downregulation, and lighter color mild to moderate up- or downregulation. Solid and dotted lines indicate direct and indirect upregulation from upstream gene A to downstream gene B, respectively. Straight lines with blunt ends indicate downregulation from upstream gene A to downstream gene B. Color images available online at

www.liebertpub.com/vim

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