The Effects of Cosmos caudatus on Structural Bone Histomorphometry in Ovariectomized Rats - PubMed (original) (raw)

The Effects of Cosmos caudatus on Structural Bone Histomorphometry in Ovariectomized Rats

Norazlina Mohamed et al. Evid Based Complement Alternat Med. 2012.

Abstract

Osteoporosis is considered a serious debilitating disease. Cosmos caudatus (ulam raja), a plant containing antioxidant compounds and minerals, may be used to treat and prevent osteoporosis. This study determines the effectiveness of C. caudatus as bone protective agent in postmenopausal osteoporosis rat model. Thirty-two female rats, aged 3 months old, were divided into 4 groups. Group one was sham operated (sham) while group two was ovariectomized. These two groups were given ionized water by forced feeding. Groups three and four were ovariectomized and given calcium 1% ad libitum and force-fed with C. caudatus at the dose of 500 mg/kg, respectively. Treatments were given six days per week for a period of eight weeks. Body weight was monitored every week and structural bone histomorphometry analyses of the femur bones were performed. Ovariectomy decreased trabecular bone volume (BV/TV), decreased trabecular number (Tb.N), and increased trabecular separation (Tb.Sp). Both calcium 1% and 500 mg/kg C. caudatus reversed the above structural bone histomorphometric parameters to normal level. C. caudatus shows better effect compared to calcium 1% on trabecular number (Tb.N) and trabecular separation (Tb.Sp). Therefore, Cosmos caudatus 500 mg/kg has the potential to act as the therapeutic agent to restore bone damage in postmenopausal women.

PubMed Disclaimer

Figures

Figure 1

Body weight of rats for all treatment groups. *Indicates significant difference (P < 0.05) compared to SO group. SO, sham operated; Ovx, ovariectomized; CC, ovariectomized and supplemented with 500 mg/kg Cosmos caudatus; Ca, ovariectomized and supplemented with 1% calcium.

Figure 2

Photomicrographs of trabecular bone. Undecalcified section (100 x magnification) shows trabecular bone (stained black) using von Kossa method. SO, sham operated; Ovx, ovariectomized; CC, ovariectomized and supplemented with 500 mg/kg Cosmos caudatus; Ca, ovariectomized and supplemented with 1% calcium.

Figure 3

Effects of Cosmos caudatus supplementation on bone volume in ovariectomized rats. Groups which share the same alphabet indicate significant difference (P < 0.05). SO, sham operated; Ovx, ovariectomized; CC, ovariectomized and supplemented with 500 mg/kg Cosmos caudatus; Ca, ovariectomized and supplemented with 1% calcium.

Figure 4

Effects of Cosmos caudatus supplementation on trabecular separation in ovariectomized rats. Groups which share the same alphabet indicate significant difference (P < 0.05). SO, sham operated; Ovx, ovariectomized; CC, ovariectomized and supplemented with 500 mg/kg Cosmos caudatus; Ca, ovariectomized and supplemented with 1% calcium.

Figure 5

Effects of Cosmos caudatus supplementation on trabecular number in ovariectomized rats. Groups which share the same alphabet indicate significant difference (P < 0.05). SO, sham operated; Ovx, ovariectomized; CC, ovariectomized and supplemented with 500 mg/kg Cosmos caudatus; Ca, ovariectomized and supplemented with 1% calcium.

Figure 6

Effects of Cosmos caudatus supplementation on trabecular thickness in ovariectomized rats. SO, sham operated; Ovx, ovariectomized; CC, ovariectomized and supplemented with 500 mg/kg Cosmos caudatus; Ca, ovariectomized and supplemented with 1% calcium.

Similar articles

• The effects of Cosmos caudatus (ulam raja) on dynamic and cellular bone histomorphometry in ovariectomized rats.
  Mohamed N, Sahhugi Z, Ramli ES, Muhammad N. Mohamed N, et al. BMC Res Notes. 2013 Jun 24;6:239. doi: 10.1186/1756-0500-6-239. BMC Res Notes. 2013. PMID: 23800238 Free PMC article.
• Potential medicinal benefits of Cosmos caudatus (Ulam Raja): A scoping review.
  Cheng SH, Barakatun-Nisak MY, Anthony J, Ismail A. Cheng SH, et al. J Res Med Sci. 2015 Oct;20(10):1000-6. doi: 10.4103/1735-1995.172796. J Res Med Sci. 2015. PMID: 26929767 Free PMC article. Review.
• The effects of Cosmos caudatus (Ulam Raja) supplementation on bone biochemical parameters in ovariectomized rats.
  Mohamed N, Yin CM, Shuid AN, Muhammad N, Babji AS, Soelaiman IN. Mohamed N, et al. Pak J Pharm Sci. 2013 Sep;26(5):1027-31. Pak J Pharm Sci. 2013. PMID: 24035963
• [Effect of Xianling Gubao capsule on bone metabolism in ovariectomized rats].
  Xi HR, Wang YY, Yang FF, Li WY, Ma HP, Chen KM. Xi HR, et al. Zhongguo Zhong Yao Za Zhi. 2018 Jul;43(13):2751-2757. doi: 10.19540/j.cnki.cjcmm.20180320.001. Zhongguo Zhong Yao Za Zhi. 2018. PMID: 30111027 Chinese.
• Estrogen Deficiency-Associated Bone Loss in the Maxilla: A Methodology to Quantify the Changes in the Maxillary Intra-radicular Alveolar Bone in an Ovariectomized Rat Osteoporosis Model.
  Du Z, Steck R, Doan N, Woodruff MA, Ivanovski S, Xiao Y. Du Z, et al. Tissue Eng Part C Methods. 2015 May;21(5):458-66. doi: 10.1089/ten.TEC.2014.0268. Epub 2014 Dec 11. Tissue Eng Part C Methods. 2015. PMID: 25315176

Cited by

• Effects of multi-deficiencies-diet on bone parameters of peripheral bone in ovariectomized mature rat.
  El Khassawna T, Böcker W, Govindarajan P, Schliefke N, Hürter B, Kampschulte M, Schlewitz G, Alt V, Lips KS, Faulenbach M, Möllmann H, Zahner D, Dürselen L, Ignatius A, Bauer N, Wenisch S, Langheinrich AC, Schnettler R, Heiss C. El Khassawna T, et al. PLoS One. 2013 Aug 16;8(8):e71665. doi: 10.1371/journal.pone.0071665. eCollection 2013. PLoS One. 2013. PMID: 23977109 Free PMC article.
• Effects of cosmos caudatus (Kenikir) antioxidant properties on bone metabolism marker in rat.
  Sari GM, Kusumawati I, Arifandi YA, Swannjo JB. Sari GM, et al. Curr Res Physiol. 2024 May 15;7:100128. doi: 10.1016/j.crphys.2024.100128. eCollection 2024. Curr Res Physiol. 2024. PMID: 38841653 Free PMC article.
• The effects of Cosmos caudatus (ulam raja) on dynamic and cellular bone histomorphometry in ovariectomized rats.
  Mohamed N, Sahhugi Z, Ramli ES, Muhammad N. Mohamed N, et al. BMC Res Notes. 2013 Jun 24;6:239. doi: 10.1186/1756-0500-6-239. BMC Res Notes. 2013. PMID: 23800238 Free PMC article.
• Potential medicinal benefits of Cosmos caudatus (Ulam Raja): A scoping review.
  Cheng SH, Barakatun-Nisak MY, Anthony J, Ismail A. Cheng SH, et al. J Res Med Sci. 2015 Oct;20(10):1000-6. doi: 10.4103/1735-1995.172796. J Res Med Sci. 2015. PMID: 26929767 Free PMC article. Review.
• Antidepressant-Like Properties of Fish Oil on Postpartum Depression-Like Rats Model: Involvement of Serotonergic System.
  Abdul Aziz NU, Chiroma SM, Mohd Moklas MA, Adenan MI, Ismail A, Hidayat Baharuldin MT. Abdul Aziz NU, et al. Brain Sci. 2020 Oct 13;10(10):733. doi: 10.3390/brainsci10100733. Brain Sci. 2020. PMID: 33066310 Free PMC article.

References

1. 1. Badeau M, Adlercreutz H, Kaihovaara P, Tikkanen MJ. Estrogen A-ring structure and antioxidative effect on lipoproteins. Journal of Steroid Biochemistry and Molecular Biology. 2005;96(3-4):271–278. - PubMed
2. 1. Lean JM, Jagger CJ, Kirstein B, Fuller K, Chambers TJ. Hydrogen peroxide is essential for estrogen-deficiency bone loss and osteoclast formation. Endocrinology. 2005;146(2):728–735. - PubMed
3. 1. Maggio D, Barabani M, Pierandrei M, et al. Marked decrease in plasma antioxidants in aged osteoporotic women: results of a cross-sectional study. Journal of Clinical Endocrinology and Metabolism. 2003;88(4):1523–1527. - PubMed
4. 1. Sontakke AN, Tare RS. A duality in the roles of reactive oxygen species with respect to bone metabolism. Clinica Chimica Acta. 2002;318(1-2):145–148. - PubMed
5. 1. Moreau MF, Chappard D, Lesourd M, Montheard JP, Basle MF. Free radicals and side products released during methylmethacrylate polimeryzation are cytotoxic for osteoblastic cells. Journal of Biomedical Materials Research. 1998;40:124–131. - PubMed

LinkOut - more resources

• Full Text Sources

  • Europe PubMed Central
  • Hindawi Limited
  • PubMed Central