Frequency of Entamoeba histolytica and Entamoeba dispar prevalence among patients with gastrointestinal complaints in Chelgerd city, southwest of Iran(*) - PubMed (original) (raw)

. 2011 Nov;16(11):1436-40.

Affiliations

Frequency of Entamoeba histolytica and Entamoeba dispar prevalence among patients with gastrointestinal complaints in Chelgerd city, southwest of Iran(*)

Nader Pestehchian et al. J Res Med Sci. 2011 Nov.

Abstract

Background: Differentiation between Entamoeba histolytica and Entamoeba dispar is very important for both clinical therapy and epidemiological studies. Although these two species are morphologically identical, they have differences in genetic, chemical specifications and pathogenicity. This study was carried out to differentiate E. histolytica from E. dispar and also to find out frequency of the two species.

Methods: Fecal samples were collected three times from 655 patients with gastrointestinal complaints (47.3% male and 52.7% female), who were referred to the primary health care centers of Chelgerd, Chaharmahal and Bakhtiary province. Samples were examined microscopically with direct smear, formalin-ethyl-acetate concentration and trichrom staining methods to distinguish E. histolytica from E. dispar complex and differentiate them from non-pathogenic intestinal amoeba. Genomic DNA was extracted from microscopy positive isolates and polymerase chain reaction (PCR) was carried out to different the two morphologically identical Entamoeba isolates.

Results: Among the 655 recruited patients, eleven subjects with E. histolytica / E. dispar isolates (1.7%) were identified by microscopy methods. Ten of the positive isolates (90.9%) were identified as E. histolytica by PCR and one isolate (9.09 %) was positive for E. dispar.

Conclusions: This study revealed that E. histolytica was more prevalent than E. dispar in the studied area. This result was different from the previously reported data in other parts of Iran.

Keywords: Entamoeba Dispar; Entamoeba Histolytica; Gastrointestinal Complaints; Iran; Polymerase Chain Reaction.

PubMed Disclaimer

Conflict of interest statement

Conflict of Interests Authors have no conflict of interests.

Figures

Figure 1

Figure 1

Agarose gel electrophoresis of positive Entamoeba dispar and Entamoeba histolytica isolates A: Lane 1, 4:100 bp marker Lane 2: Entamoeba histolytica positive control (HM-1: IMSS) with HSP1-HSP2 primers Lane 3: Entamoeba histolytica positive isolate with HSP1-HSP2 primers Lane 5: Negative control Lane 6: Entamoeba dispar positive isolate with DSP1-DSP2 primers B: Lane 1:100 bp marker Lane 2-10: Entamoeba histolytica positive isolates with HSP1-HSP2 primers

References

    1. Noor Azian MY, Lokman HS, Maslawaty MN. Use of molecular tools to distinguish Entamoeba histolytica and Entamoeba dispar infection among the aborigines in Cameron Highlands. Trop Biomed. 2006;23(1):31–6. - PubMed
    1. Qvarnstrom Y, James C, Xayavong M, Holloway BP, Visvesvara GS, Sriram R, et al. Comparison of real-time PCR protocols for differential laboratory diagnosis of amebiasis. J Clin Microbiol. 2005;43(11):5491–7. - PMC - PubMed
    1. World Health Organization. Amoebiasis. Wkly Epidemiol Rec. 1997;72:97–100.
    1. Tanyuksel M, Petri WA., Jr Laboratory diagnosis of amebiasis. Clin Microbiol Rev. 2003;16(4):713–29. - PMC - PubMed
    1. Clark CG. Entamoeba histolytica and Entamoeba dispar, the non-identical twins. In: Sterling CR, Sterling CR, Adam RD, editors. The Pathogenic Enteric Protozoa: Giardia, Entamoeba, Cryptosporidium and Cyclospora. Boston: Kluwer Academic Publishers; 2004. pp. 15–26.

LinkOut - more resources