A cell-based approach to the human proteome project - PubMed (original) (raw)

A cell-based approach to the human proteome project

Neil L Kelleher. J Am Soc Mass Spectrom. 2012 Oct.

Abstract

The general scope of a project to determine the protein molecules that comprise the cells within the human body is framed. By focusing on protein primary structure as expressed in specific cell types, this concept for a cell-based version of the Human Proteome Project (CB-HPP) is crafted in a manner analogous to the Human Genome Project while recognizing that cells provide a primary context in which to define a proteome. Several activities flow from this articulation of the HPP, which enables the definition of clear milestones and deliverables. The CB-HPP highlights major gaps in our knowledge regarding cell heterogeneity and protein isoforms, and calls for development of technology that is capable of defining all human cell types and their proteomes. The main activities will involve mapping and sorting cell types combined with the application of beyond the state-of-the art in protein mass spectrometry.

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Figure 1

Figure 1

Graphical depiction of the cell-based version of the Human Proteome Project (CB-HPP)

Figure 2

Figure 2

The levels of organization in the human body. The cell-based approach to the Human Proteome Project (CB-HPP) recognizes cell type as a primary context for mass spectrometry-based proteomics to measure the molecular complexity present in the body naturally. The CB-HPP also calls for accelerated development of new and emerging technologies to better define cell types and precisely catalogue whole protein molecules

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