Staining and embedding the whole mouse brain for electron microscopy - PubMed (original) (raw)

. 2012 Dec;9(12):1198-201.

doi: 10.1038/nmeth.2213. Epub 2012 Oct 21.

Affiliations

• PMID: 23085613
• DOI: 10.1038/nmeth.2213

Staining and embedding the whole mouse brain for electron microscopy

Shawn Mikula et al. Nat Methods. 2012 Dec.

Abstract

The development of methods for imaging large contiguous volumes with the electron microscope could allow the complete mapping of a whole mouse brain at the single-axon level. We developed a method based on prolonged immersion that enables staining and embedding of the entire mouse brain with uniform myelin staining and a moderate preservation of the tissue's ultrastructure. We tested the ability to follow myelinated axons using serial block-face electron microscopy.

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