RNA dependent RNA polymerase activity associated with the double-stranded RNA virus of Giardia lamblia - PubMed (original) (raw)
Comparative Study
. 1990 Feb 11;18(3):553-9.
doi: 10.1093/nar/18.3.553.
Affiliations
- PMID: 2308844
- PMCID: PMC333461
- DOI: 10.1093/nar/18.3.553
Free PMC article
Comparative Study
RNA dependent RNA polymerase activity associated with the double-stranded RNA virus of Giardia lamblia
T C White et al. Nucleic Acids Res. 1990.
Free PMC article
Abstract
Giardia lamblia, a parasitic protozoan, can contain a double-stranded RNA (dsRNA) virus, GLV (1). We have identified an RNA polymerase activity present specifically in cultures of GLV infected cells. This RNA polymerase activity is present in crude whole cell lysates as well as in lysates from GLV particles purified from the culture medium. The RNA polymerase has many characteristics common to other RNA polymerases (e.g. it requires divalent cations and all four ribonucleoside triphosphates), yet it is not inhibited by RNA polymerase inhibitors such as alpha-amanitin or rifampicin. The RNA polymerase activity synthesizes RNAs corresponding to one strand of the GLV genome, although under the present experimental conditions, the RNA products of the reaction are not full length viral RNAs. The in vitro products of the RNA polymerase reaction co-sediment through sucrose gradients with viral particles; and purified GLV viral particles have RNA polymerase activity. The RNA polymerase activities within and outside of infected cells closely parallel the amount of virus present during the course of viral infection. The similarities between the RNA polymerase of GLV and the polymerase associated with the dsRNA virus system of yeast are discussed.
Similar articles
- Site-specific binding of polymerase-containing particles of the Giardia lamblia double-stranded RNA virus to the viral plus-strand RNA.
Köhler S, Wang CC. Köhler S, et al. Res Virol. 1997 Sep-Oct;148(5):311-21. doi: 10.1016/s0923-2516(97)89127-3. Res Virol. 1997. PMID: 9403930 - A single-stranded RNA copy of the Giardia lamblia virus double-stranded RNA genome is present in the infected Giardia lamblia.
Furfine ES, White TC, Wang AL, Wang CC. Furfine ES, et al. Nucleic Acids Res. 1989 Sep 25;17(18):7453-67. doi: 10.1093/nar/17.18.7453. Nucleic Acids Res. 1989. PMID: 2798099 Free PMC article. - Initiation of viral RNA-dependent RNA polymerization.
van Dijk AA, Makeyev EV, Bamford DH. van Dijk AA, et al. J Gen Virol. 2004 May;85(Pt 5):1077-1093. doi: 10.1099/vir.0.19731-0. J Gen Virol. 2004. PMID: 15105525 Review. - Virion-associated protein kinases.
Hui EK. Hui EK. Cell Mol Life Sci. 2002 Jun;59(6):920-31. doi: 10.1007/s00018-002-8479-6. Cell Mol Life Sci. 2002. PMID: 12169022 Free PMC article. Review.
Cited by
- A Novel MicroRNA From the Translated Region of the Giardiavirus rdrp Gene Governs Virus Copy Number in Giardia duodenalis.
Gong P, Li X, Wu W, Cao L, Zhao P, Li X, Ren B, Li J, Zhang X. Gong P, et al. Front Microbiol. 2020 Nov 23;11:569412. doi: 10.3389/fmicb.2020.569412. eCollection 2020. Front Microbiol. 2020. PMID: 33329426 Free PMC article. - An OTU deubiquitinating enzyme in Eimeria tenella interacts with Eimeria tenella virus RDRP.
Wang P, Li J, Gong P, Wang W, Ai Y, Zhang X. Wang P, et al. Parasit Vectors. 2018 Jan 31;11(1):74. doi: 10.1186/s13071-018-2626-x. Parasit Vectors. 2018. PMID: 29386062 Free PMC article. - Three-dimensional structure of a protozoal double-stranded RNA virus that infects the enteric pathogen Giardia lamblia.
Janssen ME, Takagi Y, Parent KN, Cardone G, Nibert ML, Baker TS. Janssen ME, et al. J Virol. 2015 Jan 15;89(2):1182-94. doi: 10.1128/JVI.02745-14. Epub 2014 Nov 5. J Virol. 2015. PMID: 25378500 Free PMC article. - RNA polymerase II synthesizes antisense RNA in Plasmodium falciparum.
Militello KT, Patel V, Chessler AD, Fisher JK, Kasper JM, Gunasekera A, Wirth DF. Militello KT, et al. RNA. 2005 Apr;11(4):365-70. doi: 10.1261/rna.7940705. Epub 2005 Feb 9. RNA. 2005. PMID: 15703443 Free PMC article. - Biology of Giardia lamblia.
Adam RD. Adam RD. Clin Microbiol Rev. 2001 Jul;14(3):447-75. doi: 10.1128/CMR.14.3.447-475.2001. Clin Microbiol Rev. 2001. PMID: 11432808 Free PMC article. Review.
References
- Annu Rev Microbiol. 1980;34:49-68 - PubMed
- Nucleic Acids Res. 1980 Jul 11;8(13):2985-97 - PubMed
- Nucleic Acids Res. 1980 Jun 11;8(11):2365-75 - PubMed
- Intervirology. 1984;22(1):17-23 - PubMed
- J Virol. 1986 Mar;57(3):754-8 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources