Bacterial and archaeal diversity in sediments of west Lake Bonney, McMurdo Dry Valleys, Antarctica - PubMed (original) (raw)

Bacterial and archaeal diversity in sediments of west Lake Bonney, McMurdo Dry Valleys, Antarctica

Chao Tang et al. Appl Environ Microbiol. 2013 Feb.

Abstract

Bacterial and archaeal diversity was examined in a sediment core from Lake Bonney, Antarctica. Members of the Archaea showed both low abundance and diversity, whereas bacterial diversity was moderately high and some phyla were fairly abundant, even in geologically old samples. Microbial diversity correlated with sample texture and differed in silty and coarse samples.

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Figures

Fig 1

Fig 1

UPGMA cluster analysis of the bacterial and archaeal community structure in each WLB core section based on the DGGE banding patterns and OTU distribution of bacterial clone libraries. Dice similarity coefficients of bacterial DGGE banding patterns (A) or archaeal DGGE banding patterns (B) are shown. “N” in front of the gel label indicates nested PCR. Note that sample 5III did not yield a PCR product for the Archaea.

Fig 2

Fig 2

Distribution of bacterial phyla in each individual clone library. The y axis represents each (sub)phylum's clone coverage of each section's clone library. Low-abundance phyla not shown included the Acidobacteria (3 clones; section 4I had 2 singlets, and section 5I had 1 singlet), candidate division TM7 (section 4I had 2 singlets), the Chloroflexi (section 4I had 1 singlet), candidate division WYO (section 5I had 1 singlet), and the Lentisphaerae (section 5III had 1 singlet).

Fig 3

Fig 3

Phylogenetic analysis of major OTUs of the Bacteroidetes (A) or the Proteobacteria (B) from the WLB sediment core. Bolded sequences are from this study; one representative clone from each major OTU is shown, and in the following parentheses, the OTU name and the number of clones belonging to this particular out, separated by a comma, are listed. Both trees were rooted with Bacillus subtilis (D26185) and constructed by maximum likelihood. A mask of 702 (A) or 691 (B) nucleotides with nonambiguously aligned positions was used. Bootstrap values (100 replicates) above 60 are shown above relevant nodes. GenBank accession numbers of sequences from other studies are included.

Fig 3

Fig 3

Phylogenetic analysis of major OTUs of the Bacteroidetes (A) or the Proteobacteria (B) from the WLB sediment core. Bolded sequences are from this study; one representative clone from each major OTU is shown, and in the following parentheses, the OTU name and the number of clones belonging to this particular out, separated by a comma, are listed. Both trees were rooted with Bacillus subtilis (D26185) and constructed by maximum likelihood. A mask of 702 (A) or 691 (B) nucleotides with nonambiguously aligned positions was used. Bootstrap values (100 replicates) above 60 are shown above relevant nodes. GenBank accession numbers of sequences from other studies are included.

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References

    1. Fountain AG, Lyons WB, Burkins MB, Dana GL, Doran PT, Lewis KJ, McKnight DM, Moorhead DL, Parsons AN, Priscu JC, Wall DH, Wharton RA, Virginia RA. 1999. Physical controls on the Taylor Valley ecosystem, Antarctica. Bioscience 49:961–971
    1. Lyons W, Fountain A, Doran P, Priscu JC, Neumann K, Welch KA. 2000. Importance of landscape position and legacy: the evolution of the lakes in Taylor Valley, Antarctica. Freshw. Biol. 43:355–367
    1. Andersen DW, Wharton RA, Squyres SW. 1993. Terrigenous clastic sedimentation in Antarctic dry valley lakes, p 71–81 In Green WJ, Friedmann EI. (ed), Physical and biogeochemical processes in Antarctic lakes, vol 59 American Geophysical Union, Washington, DC
    1. Spigel RH, Priscu JC. 1998. Physical limnology of the McMurdo Dry Valleys lakes, p 153–188 In Priscu JC. (ed), Ecosystem dynamics in a polar desert: the McMurdo Dry Valleys, Antarctica, vol 72 American Geophysical Union, Washington, DC
    1. Doran P, Wharton JRA, Lyons W. 1994. Paleolimnology of the McMurdo Dry Valleys, Antarctica. J. Paleolimnol. 10:85–114 - PubMed

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