Fungal elicitor triggers rapid, transient, and specific protein phosphorylation in parsley cell suspension cultures - PubMed (original) (raw)
. 1990 Apr 15;265(11):6360-8.
Affiliations
- PMID: 2318857
Free article
Fungal elicitor triggers rapid, transient, and specific protein phosphorylation in parsley cell suspension cultures
A Dietrich et al. J Biol Chem. 1990.
Free article
Abstract
Treatment of suspension-cultured parsley (Petroselinum crispum) cells with fungal elicitor triggers rapid, transient and sequential phosphorylation of a number of proteins, as shown by electrophoretic analysis on two-dimensional gels. This response is rapidly reversed by removal of the elicitor from the medium and appears to be specific. It is not observed in cells exposed to other environmental stress factors, such as heat shock, UV irradiation or treatment with mercuric chloride. Pronase digestion of the elicitor has the same negative effect on protein phosphorylation as its previously demonstrated effect on the activation of some pathogen defense-related genes, suggesting a link between these two phenomena. Some of the changes in protein phosphorylation are among the earliest known events following elicitation. The phosphorylation of a neutral 45-kDa protein, which is found in both the microsomal and cytoplasmic fractions, can be observed as early as 1 min after the onset of elicitor treatment. The phosphorylation of a 26-kDa nuclear protein also starts increasing very early. The changes in protein phosphorylation in response to the elicitor are dependent on the presence of Ca2+ in the medium. Our data are compatible with the hypothesis that protein phosphorylation is involved in the signal transduction processes following elicitor recognition by parsley cells.
Similar articles
- Gene activation by UV light, fungal elicitor or fungal infection in Petroselinum crispum is correlated with repression of cell cycle-related genes.
Logemann E, Wu SC, Schröder J, Schmelzer E, Somssich IE, Hahlbrock K. Logemann E, et al. Plant J. 1995 Dec;8(6):865-76. doi: 10.1046/j.1365-313x.1995.8060865.x. Plant J. 1995. PMID: 8580959 - Induction of 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase activity by fungal elicitor in cultures of Petroselinum crispum.
McCue KF, Conn EE. McCue KF, et al. Proc Natl Acad Sci U S A. 1989 Oct;86(19):7374-7. doi: 10.1073/pnas.86.19.7374. Proc Natl Acad Sci U S A. 1989. PMID: 2571991 Free PMC article. - Oligopeptide elicitor-mediated defense gene activation in cultured parsley cells.
Hahlbrock K, Scheel D, Logemann E, Nürnberger T, Parniske M, Reinold S, Sacks WR, Schmelzer E. Hahlbrock K, et al. Proc Natl Acad Sci U S A. 1995 May 9;92(10):4150-7. doi: 10.1073/pnas.92.10.4150. Proc Natl Acad Sci U S A. 1995. PMID: 7753777 Free PMC article. Review. - NPP1, a Phytophthora-associated trigger of plant defense in parsley and Arabidopsis.
Fellbrich G, Romanski A, Varet A, Blume B, Brunner F, Engelhardt S, Felix G, Kemmerling B, Krzymowska M, Nürnberger T. Fellbrich G, et al. Plant J. 2002 Nov;32(3):375-90. doi: 10.1046/j.1365-313x.2002.01454.x. Plant J. 2002. PMID: 12410815 - Perception and transduction of an elicitor signal in cultured parsley cells.
Nürnberger T, Colling C, Hahlbrock K, Jabs T, Renelt A, Sacks WR, Scheel D. Nürnberger T, et al. Biochem Soc Symp. 1994;60:173-82. Biochem Soc Symp. 1994. PMID: 7639778 Review.
Cited by
- Large-scale phosphoproteome analysis in wheat seedling leaves provides evidence for extensive phosphorylation of regulatory proteins during CWMV infection.
Chen L, Yang J, Hu H, Jiang Y, Feng L, Liu J, Zhong K, Liu P, Ma Y, Chen M, Yang J. Chen L, et al. BMC Plant Biol. 2023 Nov 2;23(1):532. doi: 10.1186/s12870-023-04559-3. BMC Plant Biol. 2023. PMID: 37914991 Free PMC article. - The early response during the interaction of fungal phytopathogen and host plant.
Shen Y, Liu N, Li C, Wang X, Xu X, Chen W, Xing G, Zheng W. Shen Y, et al. Open Biol. 2017 May;7(5):170057. doi: 10.1098/rsob.170057. Open Biol. 2017. PMID: 28469008 Free PMC article. Review. - A comprehensive quantitative phosphoproteome analysis of rice in response to bacterial blight.
Hou Y, Qiu J, Tong X, Wei X, Nallamilli BR, Wu W, Huang S, Zhang J. Hou Y, et al. BMC Plant Biol. 2015 Jun 26;15:163. doi: 10.1186/s12870-015-0541-2. BMC Plant Biol. 2015. PMID: 26112675 Free PMC article. - The tomato calcium sensor Cbl10 and its interacting protein kinase Cipk6 define a signaling pathway in plant immunity.
de la Torre F, Gutiérrez-Beltrán E, Pareja-Jaime Y, Chakravarthy S, Martin GB, del Pozo O. de la Torre F, et al. Plant Cell. 2013 Jul;25(7):2748-64. doi: 10.1105/tpc.113.113530. Epub 2013 Jul 31. Plant Cell. 2013. PMID: 23903322 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Miscellaneous