The hairpin-type tail-anchored SNARE syntaxin 17 targets to autophagosomes for fusion with endosomes/lysosomes - PubMed (original) (raw)

. 2012 Dec 7;151(6):1256-69.

doi: 10.1016/j.cell.2012.11.001.

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• PMID: 23217709
• DOI: 10.1016/j.cell.2012.11.001

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The hairpin-type tail-anchored SNARE syntaxin 17 targets to autophagosomes for fusion with endosomes/lysosomes

Eisuke Itakura et al. Cell. 2012.

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Abstract

The lysosome is a degradative organelle, and its fusion with other organelles is strictly regulated. In contrast to fusion with the late endosome, the mechanisms underlying autophagosome-lysosome fusion remain unknown. Here, we identify syntaxin 17 (Stx17) as the autophagosomal SNARE required for fusion with the endosome/lysosome. Stx17 localizes to the outer membrane of completed autophagosomes but not to the isolation membrane (unclosed intermediate structures); for this reason, the lysosome does not fuse with the isolation membrane. Stx17 interacts with SNAP-29 and the endosomal/lysosomal SNARE VAMP8. Depletion of Stx17 causes accumulation of autophagosomes without degradation. Stx17 has a unique C-terminal hairpin structure mediated by two tandem transmembrane domains containing glycine zipper-like motifs, which is essential for its association with the autophagosomal membrane. These findings reveal a mechanism by which the SNARE protein is available to the completed autophagosome.

Copyright © 2012 Elsevier Inc. All rights reserved.

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Comment in

• Membrane dynamics: How lysosomes SNARE autophagosomes.
  Minton K. Minton K. Nat Rev Mol Cell Biol. 2013 Feb;14(2):65. doi: 10.1038/nrm3506. Epub 2012 Dec 19. Nat Rev Mol Cell Biol. 2013. PMID: 23249901 No abstract available.

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