Membrane curvature, lipid segregation, and structural transitions for phospholipids under dual-solvent stress - PubMed (original) (raw)

Comparative Study

. 1990 Jan 9;29(1):76-87.

doi: 10.1021/bi00453a010.

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Comparative Study

Membrane curvature, lipid segregation, and structural transitions for phospholipids under dual-solvent stress

R P Rand et al. Biochemistry. 1990.

Abstract

Amphiphiles respond both to polar and to nonpolar solvents. In this paper X-ray diffraction and osmotic stress have been used to examine the phase behavior, the structural dimensions, and the work of deforming the monolayer-lined aqueous cavities formed by mixtures of dioleoylphosphatidylethanolamine (DOPE) and dioleoylphosphatidylcholine (DOPC) as a function of the concentration of two solvents, water and tetradecane (td). In the absence of td, most PE/PC mixtures show only lamellar phases in excess water; all of these become single reverse hexagonal (HII) phases with addition of excess td. The spontaneous radius of curvature R0 of lipid monolayers, as expressed in these HII phases, is allowed by the relief of hydrocarbon chain stress by td; R0 increases with the ratio DOPC/DOPE. Mixtures with very large R0's can have water contents higher than the L alpha phases that form in the absence of td. The drive for hydration is understood in terms of the curvature energy to create large water cavities in addition to direct hydration of the polar groups. Much of the work of removing water to create hexagonal phases of radius R less than R0 goes into changing monolayer curvature rather than dehydrating polar groups. Single HII phases stressed by limited water or td show several responses. (a) The molecular area is compressed at the polar end of the molecule and expanded at the hydrocarbon ends. (b) For circularly symmetrical water cylinders, the degrees of hydrocarbon chain splaying and polar group compression are different for molecules aligned in different directions around the water cylinder. (c) A pivotal position exists along the length of the phospholipid molecule where little area change occurs as the monolayer is bent to increasing curvatures. (d) By defining R0 at the pivotal position, we find that measured energies are well fit by a quadratic bending energy, K0/2 (1/R-1/R0); the fit yields bilayer bending moduli of Kc = (1.2-1.7) X 10(-12) ergs, in good agreement with measurements from bilayer mechanics. (e) For lipid mixtures, enforced deviation of the HII monolayer from R0 is sufficiently powerful to cause demixing of the phospholipids in a way suggesting that the DOPE/DOPC ratio self-adjusts so that its R0 matches the amount of td or water available, i.e., that curvature energy is minimized.

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