Rapid procedure to determine the DNA base composition from small amounts of gram-positive bacteria - PubMed (original) (raw)
Rapid procedure to determine the DNA base composition from small amounts of gram-positive bacteria
T Ezaki et al. FEMS Microbiol Lett. 1990.
Abstract
A universal rapid procedure to determine the DNA base composition (mol% guanine + cytosine) of Gram-positive bacteria is described. Cells of Gram-positive bacteria were lysed with achromo-peptidase and the mol% G + C of their DNAs were determined by using high performance liquid chromatography. One ml of a Gram-positive bacterial suspension which matched MacFarland No. 3 standard turbidity was sufficient to determine the mol% G + C within 3 h.
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