Oral epithelial cell responses to multispecies microbial biofilms - PubMed (original) (raw)

Oral epithelial cell responses to multispecies microbial biofilms

R Peyyala et al. J Dent Res. 2013 Mar.

Abstract

This report describes the use of a novel model of multispecies biofilms to stimulate profiles of cytokines/chemokines from oral epithelial cells that contribute to local inflammation in the periodontium. Streptococcus gordonii (Sg)/S. oralis (So)/S. sanguinis (Ss) and Sg/Fusobacterium nucleatum (Fn)/Porphyromonas gingivalis (Pg) biofilms elicited significantly elevated levels of IL-1α and showed synergistic stimulatory activity compared with an additive effect of the 3 individual bacteria. Only the Sg/Actinomyces naeslundii (An)/Fn multispecies biofilms elicited IL-6 levels above those of control. IL-8 was a primary response to the Sg/An/Fn biofilms, albeit the level was not enhanced compared with a predicted composite level from the monospecies challenges. These results represent some of the first data documenting alterations in profiles of oral epithelial cell responses to multispecies biofilms.

PubMed Disclaimer

Conflict of interest statement

The authors declare no potential conflicts of interest with respect to the authorship and/or publication of this article.

Figures

Figure 1.

Levels of cytokines elicited by challenge of oral epithelial cells with multispecies biofilms (Biofilm). Mono-Biofilm denotes a composite of responses for the 3 monospecies biofilms represented in the multispecies biofilms. Each bar denotes mean level of triplicate determinations for each condition, and the vertical brackets signify 1 SD. The asterisk (*) denotes a significant difference from basal controls (RGPL-overlaid epithelial cells) at least at p < 0.05. The green horizontal line denotes mean of cells incubated in media and overlaid with RGPL absent bacteria for comparison of responses, and the light green box denotes 1 SD. The pound sign (#) denotes significant difference between multispecies biofilms and composite sum of monospecies biofilms for the 3 bacteria at least at p < 0.05. Basal epithelial cell levels of IL-1α = 245 ± 27; IL-6 = 0.64 ± 0.12; TGFα = 118 ± 16.

Figure 2.

Levels of chemokines elicited by challenge of oral epithelial cells with multispecies biofilms (Biofilm). Mono-Biofilm denotes a composite of responses for the 3 monospecies biofilms represented in the multispecies biofilms. Each bar denotes mean level of triplicate determinations for each condition, and the vertical brackets signify 1 SD. The asterisk (*) denotes a significant difference from basal controls (RGPL-overlaid epithelial cells) at least at p < 0.05. The green horizontal line denotes mean of cells incubated in media and overlaid with RGPL absent bacteria for comparison of responses, and the light green box denotes 1 SD. The pound sign (#) denotes significant difference between multispecies biofilms and composite sum of monospecies biofilms for the 3 bacteria at least at p < 0.05. Basal epithelial cell levels of IL-8 = 81 ± 11; Gro-1α (CXCL1) = 321 ± 43; Fractalkine (CX3CL1) = 56 ± 17; IP-10 (CXCL10) = 1.8 ± 0.5.

Cited by

Immunomodulatory streptococci that inhibit CXCL8 secretion and NFκB activation are common members of the oral microbiota.
Myers S, Do T, Meade JL, Tugnait A, Vernon JJ, Pistolic J, Hancock REW, Marsh PD, Trivedi HM, Chen D, Devine DA. Myers S, et al. J Med Microbiol. 2021 Mar;70(3):001329. doi: 10.1099/jmm.0.001329. Epub 2021 Mar 18. J Med Microbiol. 2021. PMID: 33734952 Free PMC article.
Multi-Omics Data Integration Reveals Key Variables Contributing to Subgingival Microbiome Dysbiosis-Induced Inflammatory Response in a Hyperglycemic Microenvironment.
Lafleur S, Bodein A, Mbuya Malaïka Mutombo J, Mathieu A, Joly Beauparlant C, Minne X, Chandad F, Droit A, Houde VP. Lafleur S, et al. Int J Mol Sci. 2023 May 16;24(10):8832. doi: 10.3390/ijms24108832. Int J Mol Sci. 2023. PMID: 37240180 Free PMC article.
Saliva-derived microcosm biofilms grown on different oral surfaces in vitro.
Li X, Shang L, Brandt BW, Buijs MJ, Roffel S, van Loveren C, Crielaard W, Gibbs S, Deng DM. Li X, et al. NPJ Biofilms Microbiomes. 2021 Sep 9;7(1):74. doi: 10.1038/s41522-021-00246-z. NPJ Biofilms Microbiomes. 2021. PMID: 34504090 Free PMC article.
The Composition of Microbiome in Larynx and the Throat Biodiversity between Laryngeal Squamous Cell Carcinoma Patients and Control Population.
Gong HL, Shi Y, Zhou L, Wu CP, Cao PY, Tao L, Xu C, Hou DS, Wang YZ. Gong HL, et al. PLoS One. 2013 Jun 18;8(6):e66476. doi: 10.1371/journal.pone.0066476. Print 2013. PLoS One. 2013. PMID: 23824228 Free PMC article.
Effects of Oral Commensal Streptococci on Porphyromonas gingivalis Invasion into Oral Epithelial Cells.
Hanel AN, Herzog HM, James MG, Cuadra GA. Hanel AN, et al. Dent J (Basel). 2020 May 2;8(2):39. doi: 10.3390/dj8020039. Dent J (Basel). 2020. PMID: 32370286 Free PMC article.

References

1. Belibasakis GN, Meier A, Guggenheim B, Bostanci N. (2011). Oral biofilm challenge regulates the RANKL-OPG system in periodontal ligament and dental pulp cells. Microb Pathog 50:6-11. - PubMed
1. Bodet C, Chandad F, Grenier D. (2006). Inflammatory responses of a macrophage/epithelial cell co-culture model to mono and mixed infections with Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia. Microbes Infect 8:27-35. - PubMed
1. Colombo AP, Boches SK, Cotton SL, Goodson JM, Kent R, Haffajee AD, et al. (2009). Comparisons of subgingival microbial profiles of refractory periodontitis, severe periodontitis, and periodontal health using the Human Oral Microbe Identification Microarray. J Periodontol 80:1421-1432. - PMC - PubMed
1. Darveau R. (2009). The oral microbial consortium’s interaction with the periodontal innate defense system. DNA Cell Biol 28:389-395. - PMC - PubMed
1. Gemmell E, Carter CL, Seymour GJ. (2001). Chemokines in human periodontal disease tissues. Clin Exp Immunol 125:134-141. - PMC - PubMed

Oral epithelial cell responses to multispecies microbial biofilms - PubMed (original) (raw)