Concentration of membrane antigens by forward transport and trapping in neuronal growth cones - PubMed (original) (raw)
Concentration of membrane antigens by forward transport and trapping in neuronal growth cones
M P Sheetz et al. Cell. 1990.
Abstract
Formation of the nervous system requires that neuronal growth cones follow specific paths and then stop at recognition signals, sensed at the growth cone's leading edge. We used antibody-coated gold particles viewed by video-enhanced differential interference contrast microscopy to observe the distribution and movement of two cell surface molecules, N-CAM and the 2A1 antigen, on growth cones of cultured cortical neurons. Gold particles are occasionally transported forward at 1-2 microns/s to the leading edge where they are trapped but continue to move. Concentration at the edge persists after cytochalasin D treatment or ATP depletion, but active movements to and along edges cease. We also observed a novel outward movement of small cytoplasmic aggregates at 1.8 microns/s in filopodia. We suggest that active forward transport and trapping involve reversible attachment of antigens to and transport along cytoskeletal elements localized to edges of growth cones.
Similar articles
- A developmentally regulated plasmalemmal antigen present in synaptosomes but not in growth cones.
Wallis I, Lasher RS, Ellis L, Siller K, Pfenninger KH. Wallis I, et al. Brain Res Dev Brain Res. 1992 Jun 19;67(2):265-78. doi: 10.1016/0165-3806(92)90227-n. Brain Res Dev Brain Res. 1992. PMID: 1511520 - L1, N-cadherin, and laminin induce distinct distribution patterns of cytoskeletal elements in growth cones.
Burden-Gulley SM, Lemmon V. Burden-Gulley SM, et al. Cell Motil Cytoskeleton. 1996;35(1):1-23. doi: 10.1002/(SICI)1097-0169(1996)35:1<1::AID-CM1>3.0.CO;2-F. Cell Motil Cytoskeleton. 1996. PMID: 8874962 - EMA: a developmentally regulated cell-surface glycoprotein of CNS neurons that is concentrated at the leading edge of growth cones.
Baumrind NL, Parkinson D, Wayne DB, Heuser JE, Pearlman AL. Baumrind NL, et al. Dev Dyn. 1992 Aug;194(4):311-25. doi: 10.1002/aja.1001940407. Dev Dyn. 1992. PMID: 1286213 - Mechanisms and molecules that control growth cone guidance.
Goodman CS. Goodman CS. Annu Rev Neurosci. 1996;19:341-77. doi: 10.1146/annurev.ne.19.030196.002013. Annu Rev Neurosci. 1996. PMID: 8833447 Review. - Cell surface antigens of cultured neuronal cells.
Akeson R. Akeson R. Curr Top Dev Biol. 1979;13 Pt 1:215-36. doi: 10.1016/s0070-2153(08)60696-1. Curr Top Dev Biol. 1979. PMID: 396117 Review. No abstract available.
Cited by
- Rapid, directed transport of DC-SIGN clusters in the plasma membrane.
Liu P, Weinreb V, Ridilla M, Betts L, Patel P, de Silva AM, Thompson NL, Jacobson K. Liu P, et al. Sci Adv. 2017 Nov 8;3(11):eaao1616. doi: 10.1126/sciadv.aao1616. eCollection 2017 Nov. Sci Adv. 2017. PMID: 29134199 Free PMC article. - Mechanism of Axonal Contractility in Embryonic Drosophila Motor Neurons In Vivo.
Tofangchi A, Fan A, Saif MTA. Tofangchi A, et al. Biophys J. 2016 Oct 4;111(7):1519-1527. doi: 10.1016/j.bpj.2016.08.024. Biophys J. 2016. PMID: 27705774 Free PMC article. - Computational estimates of membrane flow and tension gradient in motile cells.
Fogelson B, Mogilner A. Fogelson B, et al. PLoS One. 2014 Jan 17;9(1):e84524. doi: 10.1371/journal.pone.0084524. eCollection 2014. PLoS One. 2014. PMID: 24465414 Free PMC article. - Actin-independent behavior and membrane deformation exhibited by the four-transmembrane protein M6a.
Sato Y, Watanabe N, Fukushima N, Mita S, Hirata T. Sato Y, et al. PLoS One. 2011;6(12):e26702. doi: 10.1371/journal.pone.0026702. Epub 2011 Dec 5. PLoS One. 2011. PMID: 22162747 Free PMC article. - Directional spread of surface-associated retroviruses regulated by differential virus-cell interactions.
Sherer NM, Jin J, Mothes W. Sherer NM, et al. J Virol. 2010 Apr;84(7):3248-58. doi: 10.1128/JVI.02155-09. Epub 2010 Jan 20. J Virol. 2010. PMID: 20089647 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials
Miscellaneous