Gene duplication in the evolution of the two complementing domains of gram-negative bacterial tetracycline efflux proteins - PubMed (original) (raw)
Comparative Study
Gene duplication in the evolution of the two complementing domains of gram-negative bacterial tetracycline efflux proteins
R A Rubin et al. Gene. 1990.
Abstract
The resistance of Gram- bacteria to the broad-spectrum antibiotic tetracycline (Tc) results from energy-dependent drug efflux mediated by the tet gene product, the cytoplasmic membrane Tet protein. Amino acid (aa) sequences deduced from total tet nucleotide sequences of three different resistance determinants (classes A, B and C) indicate that the protein products [Tet(A), Tet(B), and Tet(C)] share a common ancestor. Hydropathic analysis of Tet sequences predicts twelve transmembrane segments in each protein, with six occurring in each half of the molecule. More importantly, the linear distributions of these segments in the N- and C-terminal halves are nearly identical, suggesting that the two halves of each Tet protein are related by a process of tandem gene duplication and divergence. Indeed, a variable but significant conservation of sequence was detected among the N- and C-terminal halves for all possible comparisons of the three proteins. Such conservation was not observed within other prokaryotic integral membrane proteins or when other prokaryotic proteins were compared to Tet halves. Similarity, both in sequence and in predicted transmembrane structural organization, strongly suggests that a common ancestor of Tet(A), Tet(B), and Tet(C) arose by duplication of a gene reading frame specifying a transmembrane protein of approximately 200 aa residues. The two halves of Tet proteins correspond to the two domains, alpha and beta, which have distinct, complementary roles in Tc efflux. Nevertheless, selective constraints to function in the cytoplasmic membrane have apparently led to maintenance of similar patterns of secondary structural organization in these complementary domains.
Similar articles
- Interdomain hybrid Tet proteins confer tetracycline resistance only when they are derived from closely related members of the tet gene family.
Rubin RA, Levy SB. Rubin RA, et al. J Bacteriol. 1990 May;172(5):2303-12. doi: 10.1128/jb.172.5.2303-2312.1990. J Bacteriol. 1990. PMID: 2185211 Free PMC article. - Tet protein domains interact productively to mediate tetracycline resistance when present on separate polypeptides.
Rubin RA, Levy SB. Rubin RA, et al. J Bacteriol. 1991 Jul;173(14):4503-9. doi: 10.1128/jb.173.14.4503-4509.1991. J Bacteriol. 1991. PMID: 2066343 Free PMC article. - TetZ, a new tetracycline resistance determinant discovered in gram-positive bacteria, shows high homology to gram-negative regulated efflux systems.
Tauch A, Pühler A, Kalinowski J, Thierbach G. Tauch A, et al. Plasmid. 2000 Nov;44(3):285-91. doi: 10.1006/plas.2000.1489. Plasmid. 2000. PMID: 11078655 - Mechanisms underlying expression of Tn10 encoded tetracycline resistance.
Hillen W, Berens C. Hillen W, et al. Annu Rev Microbiol. 1994;48:345-69. doi: 10.1146/annurev.mi.48.100194.002021. Annu Rev Microbiol. 1994. PMID: 7826010 Review. - Functions of tetracycline efflux proteins that do not involve tetracycline.
Krulwich TA, Jin J, Guffanti AA, Bechhofer H. Krulwich TA, et al. J Mol Microbiol Biotechnol. 2001 Apr;3(2):237-46. J Mol Microbiol Biotechnol. 2001. PMID: 11321579 Review.
Cited by
- The Role of Gene Elongation in the Evolution of Histidine Biosynthetic Genes.
Del Duca S, Chioccioli S, Vassallo A, Castronovo LM, Fani R. Del Duca S, et al. Microorganisms. 2020 May 13;8(5):732. doi: 10.3390/microorganisms8050732. Microorganisms. 2020. PMID: 32414216 Free PMC article. - The Structure and Function of OxlT, the Oxalate Transporter of Oxalobacter formigenes.
Iyalomhe O, Khantwal CM, Kang DC. Iyalomhe O, et al. J Membr Biol. 2015 Aug;248(4):641-50. doi: 10.1007/s00232-014-9728-y. Epub 2014 Sep 16. J Membr Biol. 2015. PMID: 25224873 Review. - A maize defense-inducible gene is a major facilitator superfamily member related to bacterial multidrug resistance efflux antiporters.
Simmons CR, Fridlender M, Navarro PA, Yalpani N. Simmons CR, et al. Plant Mol Biol. 2003 May;52(2):433-46. doi: 10.1023/a:1023982704901. Plant Mol Biol. 2003. PMID: 12856948 - Regulation of phosphate acquisition in Saccharomyces cerevisiae.
Persson BL, Lagerstedt JO, Pratt JR, Pattison-Granberg J, Lundh K, Shokrollahzadeh S, Lundh F. Persson BL, et al. Curr Genet. 2003 Jul;43(4):225-44. doi: 10.1007/s00294-003-0400-9. Epub 2003 May 10. Curr Genet. 2003. PMID: 12740714 Review. - Structural model for 12-helix transporters belonging to the major facilitator superfamily.
Hirai T, Heymann JA, Maloney PC, Subramaniam S. Hirai T, et al. J Bacteriol. 2003 Mar;185(5):1712-8. doi: 10.1128/JB.185.5.1712-1718.2003. J Bacteriol. 2003. PMID: 12591890 Free PMC article.