Synthetic circuits integrating logic and memory in living cells - PubMed (original) (raw)
doi: 10.1038/nbt.2510. Epub 2013 Feb 10.
Affiliations
- PMID: 23396014
- DOI: 10.1038/nbt.2510
Synthetic circuits integrating logic and memory in living cells
Piro Siuti et al. Nat Biotechnol. 2013 May.
Abstract
Logic and memory are essential functions of circuits that generate complex, state-dependent responses. Here we describe a strategy for efficiently assembling synthetic genetic circuits that use recombinases to implement Boolean logic functions with stable DNA-encoded memory of events. Application of this strategy allowed us to create all 16 two-input Boolean logic functions in living Escherichia coli cells without requiring cascades comprising multiple logic gates. We demonstrate long-term maintenance of memory for at least 90 cell generations and the ability to interrogate the states of these synthetic devices with fluorescent reporters and PCR. Using this approach we created two-bit digital-to-analog converters, which should be useful in biotechnology applications for encoding multiple stable gene expression outputs using transient inputs of inducers. We envision that this integrated logic and memory system will enable the implementation of complex cellular state machines, behaviors and pathways for therapeutic, diagnostic and basic science applications.
Comment in
- Endowing cells with logic and memory.
Maranhao AC, Ellington AD. Maranhao AC, et al. Nat Biotechnol. 2013 May;31(5):413-5. doi: 10.1038/nbt.2573. Nat Biotechnol. 2013. PMID: 23657394 No abstract available.
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