Sequencing ancient calcified dental plaque shows changes in oral microbiota with dietary shifts of the Neolithic and Industrial revolutions - PubMed (original) (raw)

Figure 1. Phylum level microbial composition of the ancient dental calculus deposits

This is similar to modern oral samples, and distinct from non-template controls (EBC), ancient human teeth and environmental samples. The phylum frequencies for the V3 region are presented for the ancient calculus samples (LBK; Linear Pottery Culture, BB; Bell Beaker), modern oral samples, which included pyrosequenced (calculus, plaque and saliva) and cloned (plaque,,) data, non-template controls (or extraction blanks), ancient human teeth and environmental samples (freshwater, sediments and soils-) (Supplementary Table 1). Phylum frequencies from the HOMD were generated from partial and full-length sequences of the 16S rRNA gene. The phyla with a frequency < 1% include: ABY1_OD1, AD3, Armatimonadetes, BRC1, CCM11b, Chlamydiae, Chlorobi, Cyanobacteria, Elusimicrobia, Euryarchaeota, Fibrobacteres, GAL15, Gemmatimonadetes, GN02, GN04, GOUTA4, KSB1, Lentisphaerae, NC10, Nitrospirae, NKB19, OP11, OP3, OP9, PAUC34f, Planctomycetes, SBR1093, SC3, SC4, SM2F11, SPAM, Spirochaetes, SR1, Tenericutes, Thermi, TM6, Verrucomicrobia, WPS-2, WS3 and ZB2.