Transcription of the procyclic acidic repetitive protein genes of Trypanosoma brucei - PubMed (original) (raw)
Comparative Study
Transcription of the procyclic acidic repetitive protein genes of Trypanosoma brucei
C E Clayton et al. Mol Cell Biol. 1990 Jun.
Abstract
The procyclic acidic repetitive protein (parp) genes of Trypanosoma brucei encode a small family of abundant surface proteins whose expression is restricted to the procyclic form of the parasite. They are found at two unlinked loci, parpA and parpB; transcription of both loci is developmentally regulated. The region of homology upstream of the A and B parp genes is only 640 base pairs long and may contain sequences responsible for transcriptional initiation and regulation. Transcription upstream of this putative promoter region is not developmentally regulated and is much less active than that of the parp genes; the polymerase responsible is inhibited by alpha-amanitin, whereas that transcribing the parp genes is not. Transcription of the parp genes is strongly stimulated by low levels of UV irradiation. The putative parp promoter, when placed upstream of the chloramphenicol acetyltransferase gene, is sufficient to cause production of chloramphenicol acetyltransferase in a T. brucei DNA transformation assay. Taken together, these results suggest that a promoter for an alpha-amanitin-resistant RNA polymerase lies less than 600 nucleotides upstream of the parp genes.
Similar articles
- Procyclic acidic repetitive protein (PARP) genes located in an unusually small alpha-amanitin-resistant transcription unit: PARP promoter activity assayed by transient DNA transfection of Trypanosoma brucei.
Rudenko G, Le Blancq S, Smith J, Lee MG, Rattray A, Van der Ploeg LH. Rudenko G, et al. Mol Cell Biol. 1990 Jul;10(7):3492-504. doi: 10.1128/mcb.10.7.3492-3504.1990. Mol Cell Biol. 1990. PMID: 1694012 Free PMC article. - Anatomy of the parp gene promoter of Trypanosoma brucei.
Sherman DR, Janz L, Hug M, Clayton C. Sherman DR, et al. EMBO J. 1991 Nov;10(11):3379-86. doi: 10.1002/j.1460-2075.1991.tb04902.x. EMBO J. 1991. PMID: 1840521 Free PMC article. - Regulation of vsg expression site transcription and switching in Trypanosoma brucei.
Cross GA, Wirtz LE, Navarro M. Cross GA, et al. Mol Biochem Parasitol. 1998 Mar 1;91(1):77-91. doi: 10.1016/s0166-6851(97)00186-2. Mol Biochem Parasitol. 1998. PMID: 9574927 Review. - The glycosylation of the variant surface glycoproteins and procyclic acidic repetitive proteins of Trypanosoma brucei.
Mehlert A, Zitzmann N, Richardson JM, Treumann A, Ferguson MA. Mehlert A, et al. Mol Biochem Parasitol. 1998 Mar 1;91(1):145-52. doi: 10.1016/s0166-6851(97)00187-4. Mol Biochem Parasitol. 1998. PMID: 9574932 Review.
Cited by
- Genomic Occupancy of the Bromodomain Protein Bdf3 Is Dynamic during Differentiation of African Trypanosomes from Bloodstream to Procyclic Forms.
Ashby E, Paddock L, Betts HL, Liao J, Miller G, Porter A, Rollosson LM, Saada C, Tang E, Wade SJ, Hardin J, Schulz D. Ashby E, et al. mSphere. 2022 Jun 29;7(3):e0002322. doi: 10.1128/msphere.00023-22. Epub 2022 Jun 1. mSphere. 2022. PMID: 35642518 Free PMC article. - Trypanosoma brucei CYP51: Essentiality and Targeting Therapy in an Experimental Model.
Dauchy FA, Bonhivers M, Landrein N, Dacheux D, Courtois P, Lauruol F, Daulouède S, Vincendeau P, Robinson DR. Dauchy FA, et al. PLoS Negl Trop Dis. 2016 Nov 17;10(11):e0005125. doi: 10.1371/journal.pntd.0005125. eCollection 2016 Nov. PLoS Negl Trop Dis. 2016. PMID: 27855164 Free PMC article. - Ribosomal RNA gene transcription in trypanosomes.
Hernández R, Cevallos AM. Hernández R, et al. Parasitol Res. 2014 Jul;113(7):2415-24. doi: 10.1007/s00436-014-3940-7. Epub 2014 May 15. Parasitol Res. 2014. PMID: 24828347 Review. - Characterization of two different Asf1 histone chaperones with distinct cellular localizations and functions in Trypanosoma brucei.
Pascoalino B, Dindar G, Vieira-da-Rocha JP, Machado CR, Janzen CJ, Schenkman S. Pascoalino B, et al. Nucleic Acids Res. 2014 Mar;42(5):2906-18. doi: 10.1093/nar/gkt1267. Epub 2013 Dec 9. Nucleic Acids Res. 2014. PMID: 24322299 Free PMC article. - Genetic engineering of Trypanosoma (Dutonella) vivax and in vitro differentiation under axenic conditions.
D'Archivio S, Medina M, Cosson A, Chamond N, Rotureau B, Minoprio P, Goyard S. D'Archivio S, et al. PLoS Negl Trop Dis. 2011 Dec;5(12):e1461. doi: 10.1371/journal.pntd.0001461. Epub 2011 Dec 27. PLoS Negl Trop Dis. 2011. PMID: 22216367 Free PMC article.
References
- EMBO J. 1988 May;7(5):1431-7 - PubMed
- EMBO J. 1989 Sep;8(9):2633-8 - PubMed
- J Biol Chem. 1988 Oct 15;263(29):15071-5 - PubMed
- Mol Cell Biol. 1988 Oct;8(10):4055-62 - PubMed
- EMBO J. 1988 Aug;7(8):2539-45 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources