Structural biology in situ--the potential of subtomogram averaging - PubMed (original) (raw)
Review
Structural biology in situ--the potential of subtomogram averaging
John A G Briggs. Curr Opin Struct Biol. 2013 Apr.
Abstract
Cryo-electron tomography provides low-resolution 3D views of cells, organelles, or viruses. Macromolecular complexes present in multiple copies can be subsequently identified within the 3D reconstruction (the tomogram), computationally extracted, and averaged to obtain higher resolution 3D structures, as well as a map of their spatial distribution. This method, called subtomogram averaging or subvolume averaging, allows structures of macromolecular complexes to be resolved in situ. Recent applications have provided in situ structural data at resolutions of 2-4 nm on samples including polysomes, nuclear pores, vesicle coats, and viral surface proteins. Here I describe the method and discuss limitations, advances and recent applications. I speculate how the method will solve more structures at higher resolution, allowing in situ structural biology.
Copyright © 2013 Elsevier Ltd. All rights reserved.
Similar articles
- Cryo-Electron Tomography and Subtomogram Averaging.
Wan W, Briggs JA. Wan W, et al. Methods Enzymol. 2016;579:329-67. doi: 10.1016/bs.mie.2016.04.014. Epub 2016 Jun 22. Methods Enzymol. 2016. PMID: 27572733 Review. - Subtomogram averaging from cryo-electron tomograms.
Leigh KE, Navarro PP, Scaramuzza S, Chen W, Zhang Y, Castaño-Díez D, Kudryashev M. Leigh KE, et al. Methods Cell Biol. 2019;152:217-259. doi: 10.1016/bs.mcb.2019.04.003. Epub 2019 May 15. Methods Cell Biol. 2019. PMID: 31326022 Review. - Determination of protein structure at 8.5Å resolution using cryo-electron tomography and sub-tomogram averaging.
Schur FK, Hagen WJ, de Marco A, Briggs JA. Schur FK, et al. J Struct Biol. 2013 Dec;184(3):394-400. doi: 10.1016/j.jsb.2013.10.015. Epub 2013 Oct 30. J Struct Biol. 2013. PMID: 24184468 - Progressive Stochastic Reconstruction Technique (PSRT) for cryo electron tomography.
Turoňová B, Marsalek L, Davidovič T, Slusallek P. Turoňová B, et al. J Struct Biol. 2015 Mar;189(3):195-206. doi: 10.1016/j.jsb.2015.01.011. Epub 2015 Feb 4. J Struct Biol. 2015. PMID: 25659894 - Implementation of a cryo-electron tomography tilt-scheme optimized for high resolution subtomogram averaging.
Hagen WJH, Wan W, Briggs JAG. Hagen WJH, et al. J Struct Biol. 2017 Feb;197(2):191-198. doi: 10.1016/j.jsb.2016.06.007. Epub 2016 Jun 14. J Struct Biol. 2017. PMID: 27313000 Free PMC article.
Cited by
- Few shot domain adaptation for in situ macromolecule structural classification in cryoelectron tomograms.
Yu L, Li R, Zeng X, Wang H, Jin J, Ge Y, Jiang R, Xu M. Yu L, et al. Bioinformatics. 2021 Apr 19;37(2):185-191. doi: 10.1093/bioinformatics/btaa671. Bioinformatics. 2021. PMID: 32722755 Free PMC article. - Multi-task Learning for Macromolecule Classification, Segmentation and Coarse Structural Recovery in Cryo-Tomography.
Liu C, Zeng X, Wang KW, Guo Q, Xu M. Liu C, et al. BMVC. 2018 Sep;2018:1007. BMVC. 2018. PMID: 36951799 Free PMC article. - (Cryo)Transmission Electron Microscopy of Phospholipid Model Membranes Interacting with Amphiphilic and Polyphilic Molecules.
Meister A, Blume A. Meister A, et al. Polymers (Basel). 2017 Oct 19;9(10):521. doi: 10.3390/polym9100521. Polymers (Basel). 2017. PMID: 30965829 Free PMC article. Review. - Structure of the immature HIV-1 capsid in intact virus particles at 8.8 Å resolution.
Schur FK, Hagen WJ, Rumlová M, Ruml T, Müller B, Kräusslich HG, Briggs JA. Schur FK, et al. Nature. 2015 Jan 22;517(7535):505-8. doi: 10.1038/nature13838. Epub 2014 Nov 2. Nature. 2015. PMID: 25363765 - Structural differences observed in arboviruses of the alphavirus and flavivirus genera.
Hernandez R, Brown DT, Paredes A. Hernandez R, et al. Adv Virol. 2014;2014:259382. doi: 10.1155/2014/259382. Epub 2014 Sep 16. Adv Virol. 2014. PMID: 25309597 Free PMC article. Review.
Publication types
MeSH terms
LinkOut - more resources
Full Text Sources
Other Literature Sources