Kinetics, stoichiometry and role of the Na-Ca exchange mechanism in isolated cardiac myocytes - PubMed (original) (raw)
. 1990 Jun 14;345(6276):618-21.
doi: 10.1038/345618a0.
Affiliations
- PMID: 2348872
- DOI: 10.1038/345618a0
Kinetics, stoichiometry and role of the Na-Ca exchange mechanism in isolated cardiac myocytes
L M Crespo et al. Nature. 1990.
Abstract
Compelling evidence has existed for more than a decade for a sodium/calcium (Na-Ca) exchange mechanism in the surface membrane of mammalian heart muscle cells which exchanges about three sodium ions for each calcium ion. Although it is known that cardiac muscle contraction is regulated by a transient increase in intracellular calcium ([Ca2+]i) triggered by the action potential, the contribution of the Na-Ca exchanger to the [Ca2+]i transient and to calcium extrusion during rest is unclear. To clarify these questions, changes in [Ca2+]i were measured with indo-1 in single cardiac myocytes which were voltage clamped and dialysed with a physiological level of sodium. We find that Ca entry through the Na-Ca exchanger is too slow to affect markedly the rate of rise of the normal [Ca2+]i transient. On repolarization, Ca extrusion by the exchanger causes [Ca2+]i to decline with a time constant of 0.5 s at -80 mV. The rate of decline can be slowed e-fold with a 77-mV depolarization. Calcium extrusion by the exchanger can account for about 15% of the rate of decline of the [Ca2+]i transient (the remainder being calcium resequestration by the sarcoplasmic reticulum (SR]. The ability of the cell to extrude calcium was greatly reduced on inhibiting the exchanger by removing external sodium, which itself led to an increase in resting [Ca2+]i. This finding is in contrast to the suggestion that calcium extrusion at rest is mediated mainly by a sarcolemmal Ca-ATPase.(ABSTRACT TRUNCATED AT 250 WORDS)
Similar articles
- Intracellular Ca transients in rat cardiac myocytes: role of Na-Ca exchange in excitation-contraction coupling.
Bers DM, Lederer WJ, Berlin JR. Bers DM, et al. Am J Physiol. 1990 May;258(5 Pt 1):C944-54. doi: 10.1152/ajpcell.1990.258.5.C944. Am J Physiol. 1990. PMID: 2333986 - Sodium-calcium exchange in mammalian heart: current-voltage relation and intracellular calcium concentration.
Wier WG, Beuckelmann DJ. Wier WG, et al. Mol Cell Biochem. 1989 Sep 7;89(2):97-102. doi: 10.1007/BF00220759. Mol Cell Biochem. 1989. PMID: 2554126 - Relaxation in ferret ventricular myocytes: unusual interplay among calcium transport systems.
Bassani RA, Bassani JW, Bers DM. Bassani RA, et al. J Physiol. 1994 Apr 15;476(2):295-308. doi: 10.1113/jphysiol.1994.sp020131. J Physiol. 1994. PMID: 8046644 Free PMC article. - Na(+)--Ca2+ exchange in the regulation of cardiac excitation-contraction coupling.
Reuter H, Pott C, Goldhaber JI, Henderson SA, Philipson KD, Schwinger RH. Reuter H, et al. Cardiovasc Res. 2005 Aug 1;67(2):198-207. doi: 10.1016/j.cardiores.2005.04.031. Cardiovasc Res. 2005. PMID: 15935336 Review. - Sodium/calcium exchange in ventricular muscle.
McGuigan JA, Blatter LA. McGuigan JA, et al. Experientia. 1987 Dec 1;43(11-12):1140-5. doi: 10.1007/BF01945512. Experientia. 1987. PMID: 2446907 Review.
Cited by
- Structural insight into the allosteric inhibition of human sodium-calcium exchanger NCX1 by XIP and SEA0400.
Dong Y, Yu Z, Li Y, Huang B, Bai Q, Gao Y, Chen Q, Li N, He L, Zhao Y. Dong Y, et al. EMBO J. 2024 Jan;43(1):14-31. doi: 10.1038/s44318-023-00013-0. Epub 2023 Dec 15. EMBO J. 2024. PMID: 38177313 Free PMC article. - Cirrhotic Cardiomyopathy: The Interplay Between Liver and Heart.
Kalluru R, Gadde S, Chikatimalla R, Dasaradhan T, Koneti J, Cherukuri SP. Kalluru R, et al. Cureus. 2022 Aug 13;14(8):e27969. doi: 10.7759/cureus.27969. eCollection 2022 Aug. Cureus. 2022. PMID: 36120195 Free PMC article. Review. - The Role of Adenosine A2b Receptor in Mediating the Cardioprotection of Electroacupuncture Pretreatment via Influencing Ca2+ Key Regulators.
Dai QF, Gao JH, Xin JJ, Liu Q, Jing XH, Yu XC. Dai QF, et al. Evid Based Complement Alternat Med. 2019 Dec 2;2019:6721286. doi: 10.1155/2019/6721286. eCollection 2019. Evid Based Complement Alternat Med. 2019. PMID: 31885657 Free PMC article. - Assessing SSRIs' effects on fetal cardiomyocytes utilizing placenta-fetus model.
Arumugasaamy N, Hurley-Novatny A, Lembong J, Kim PCW, Fisher JP. Arumugasaamy N, et al. Acta Biomater. 2019 Nov;99:258-268. doi: 10.1016/j.actbio.2019.09.019. Epub 2019 Sep 16. Acta Biomater. 2019. PMID: 31536839 Free PMC article. - Late Ca2+ Sparks and Ripples During the Systolic Ca2+ Transient in Heart Muscle Cells.
Fowler ED, Kong CHT, Hancox JC, Cannell MB. Fowler ED, et al. Circ Res. 2018 Feb 2;122(3):473-478. doi: 10.1161/CIRCRESAHA.117.312257. Epub 2017 Dec 27. Circ Res. 2018. PMID: 29282211 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
Research Materials
Miscellaneous