Structural and functional studies of the 252 kDa nucleoporin ELYS reveal distinct roles for its three tethered domains - PubMed (original) (raw)

Structural and functional studies of the 252 kDa nucleoporin ELYS reveal distinct roles for its three tethered domains

Silvija Bilokapic et al. Structure. 2013.

Abstract

In metazoa, the nuclear envelope (NE), together with the embedded nuclear pore complexes (NPCs), breaks down and reassembles during cell division. It is suggested that ELYS, a nucleoporin, binds to chromatin in an initial step of postmitotic NPC assembly and subsequently recruits the essential Y-subcomplex, the major scaffolding unit of the NPC. Here, we show that ELYS contains three domains: an N-terminal β-propeller domain, a central α-helical domain, and a C-terminal disordered region. While the disordered region is responsible for the interactions with chromatin, the two preceding domains synergistically mediate tethering to the NPC. We present the crystal structure of the seven-bladed β-propeller domain at 1.9 Å resolution. Analysis of the β-propeller surface reveals the regions that are required for NPC anchorage. We discuss the possible roles of ELYS in the context of the NPC scaffold architecture.

Copyright © 2013 Elsevier Ltd. All rights reserved.

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Figures

Figure 1

Figure 1. Domain organization of ELYS

Secondary structure prediction methods suggest three domains in higher eukaryotes, with an N-terminal β-stranded domain (NTD), a central α-helical domain (CHD) and a disordered C-terminal region (CTR). In unicellular eukaryotes, i.e. S. pombe, only the CHD is conserved in ELYS homologs. The constructs used for crystallization are denoted with black bars. The residue numbering is based on UniProt entries Q8CJF7 (M. musculus), Q8WYP5 (H. sapiens), and O94384 (S. pombe).

Figure 2

Figure 2. In vivo localization of metazoan ELYS domains

(A) Domain organization of the GFP-labeled mELYS constructs and their localization in HeLa cells. Full-length mELYS (residues 1–2243) shows nuclear rim staining and intra-nuclear puncta. The construct containing both structured domains (NTD and CHD, residues 1–1018) also localize to the nuclear rim, while the disordered region (CTR, residues 1019–2243) shows diffuse nuclear GFP-signal. (B) Domain organization of the GFP-labeled hELYS constructs and their localization in HeLa cells. The constructs containing only the NTD (residues 1–494) or the CHD (residues 495–1018) fail to localize to the nuclear rim in HeLa cells, showing that in metazoa both domains (residues 1–1018) are required for interactions with the NPC. (C) Fusion of the classical SV40 NLS to the C terminus of the hELYS NTD induces nuclear translocation but the domain does not concentrate at the nuclear rim.

Figure 3

Figure 3. Structural analysis of mELYS NTD

(A) Cartoon representation of the mELYS β–propeller domain with the blades labeled from 1 to 7. As a reference, the strands of blade 3 are labeled A–D, with the non-canonical 5th strand labeled D′. The β–propeller blades are colored in blue, loops are in white and the secondary structure insertions within the loops are shown in orange. (B) Comparison of the β–propeller blades reveals long loops that decorate the structural core and contribute about 40% of the total mass of the 494 residue mELYS NTD. See also Figure S1.

Figure 4

Figure 4. Conserved features of the mELYS β–propeller

(A) Cartoon representation of mELYS NTD from three different perspectives, related by rotation around the vertical axis as indicated. Conservation within the mELYS NTD is gradient-colored from gray (not conserved) to dark orange (most conserved) based on the alignment in Figure S3. The majority of conserved residues are structurally important as they build the core scaffold of the β–propeller. (B) Surface representation showing conservation of residues on the β–propeller periphery. Color scheme as in A. The two conserved loops building the NPC binding interface are outlined with a black dotted line. See also Figure S3.

Figure 5

Figure 5. Mutations in the conserved β–propeller loops abolish proper NPC localization of mELYS

(A) Cartoon representation of mELYS NTD with the residues in the conserved loops shown as sticks. Loop1 residues are highlighted in yellow and loop2 residues in pink. Residues forming a hydrophobic pocket beneath loop2 are shown in gray. (B) Domain organization of the GFP-tagged mELYS mutants with the positions of the mutations indicated by red bars (loop deletions or multiple mutations) or red arrowheads (single mutations). (C) Confocal fluorescence microscopy of HeLa cells expressing GFP-tagged mELYS proteins. Wild-type mELYS properly localizes to the nuclear envelope. Deletion of the disordered loop3 (control, residues 458–476), as well as milder mutations within the binding region show nuclear rim staining. The mELYSΔloop1/Y284S and mELYSloop2mut mutants abolish nuclear rim localization. See also Figure S4.

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