Detection of Alphacoronavirus in velvety free-tailed bats (Molossus molossus) and Brazilian free-tailed bats (Tadarida brasiliensis) from urban area of Southern Brazil - PubMed (original) (raw)

Detection of Alphacoronavirus in velvety free-tailed bats (Molossus molossus) and Brazilian free-tailed bats (Tadarida brasiliensis) from urban area of Southern Brazil

Francisco Esmaile de Sales Lima et al. Virus Genes. 2013 Aug.

Abstract

A survey was carried out in search for bat coronaviruses in an urban maternity roost of about 500 specimens of two species of insectivorous bats, Molossus molossus and Tadarida brasiliensis, in Southern Brazil. Twenty-nine out of 150 pooled fecal samples tested positive by reverse transcription-PCR contained fragments of the RNA-dependent RNA polymerase gene of coronavirus-related viruses. The sequences clustered along with bat alphacoronaviruses, forming a subcluster within this group. Our findings point to the need for risk assessment and continued surveillance of coronavirus infections of bats in Brazil.

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Figures

Fig. 1

Fig. 1

Phylogenetic relationship between the nucleotide sequences corresponding to a portion of 440 bp of the RNA-dependent RNA polymerase gene amplified from insectivorous bat species in Porto Alegre, RS, Brazil. Sequences from other alpha and betacoronaviruses were obtained from GenBanK (accession numbers in the figure). The tree was generated based on the neighbor joining method in the MEGA program. The nucleotide sequence of the equivalent genome fragment of SARS-CoV was included as outgroup

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