LC-MS/MS determination of pogostone in rat plasma and its application in pharmacokinetic studies - PubMed (original) (raw)

doi: 10.1002/bmc.2897. Epub 2013 Mar 18.

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LC-MS/MS determination of pogostone in rat plasma and its application in pharmacokinetic studies

Haiming Chen et al. Biomed Chromatogr. 2013 Sep.

Abstract

Pogostone is an important constituent of Pogostemon cablin (Blanco) Benth., and possesses various known bioactivities. A rapid, simple and sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) method was developed for the analysis of pogostone in rat plasma using chrysophanol as internal standard (IS). The analytes were extracted with methanol and separated using a reversed-phase YMC-UltraHT Pro C18 column. Elution was achieved with a mobile phase consisting of methanol-water (75:25, v/v) for 5 min at a flow rate of 400 μL/min. The precursor/product transitions (m/z) under MS/MS detection with negative electrospray ionization (ESI) were 223.0 → 139.0 and 253.1 → 224.9 for pogostone and IS, respectively. The calibration curve was linear over the concentration range 0.05-160 µg/mL (r = 0.9996). The intra- and inter-day accuracy and precision were within ±10%. The validated method was successfully applied to the preclinical pharmacokinetic investigation of pogostone in rats after intravenous (5, 10 and 20 mg/kg) and oral administration (5, 10 and 20 mg/kg). Finally, the oral absolute bioavailability of pogostone in rats was calculated to be 70.39, 78.18 and 83.99% for 5, 10 and 20 mg/kg, respectively.

Keywords: LC-MS/MS; pharmacokinetics; pogostone; rat plasma.

Copyright © 2013 John Wiley & Sons, Ltd.

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