The bulk of the peripheral B-cell pool in mice is stable and not rapidly renewed from the bone marrow - PubMed (original) (raw)
The bulk of the peripheral B-cell pool in mice is stable and not rapidly renewed from the bone marrow
I Förster et al. Proc Natl Acad Sci U S A. 1990 Jun.
Abstract
We have reevaluated the in vivo lifespan of B lymphocytes based on incorporation of the thymidine analogue 5-bromo-2'-deoxyuridine into the DNA of dividing cells. To exclude potential alterations in the turnover of B-lineage cells due to the bromodeoxyuridine incorporation, we performed pulse-chase experiments comparing the appearance and disappearance of bromodeoxyuridine-labeled cells over long periods of time. The data consistently show that more than two-thirds of splenic B cells in adult (greater than 2 months old) mice have a lifetime of several weeks or months, whereas a more rapid turnover takes place in young (4 week old) mice. Thus, the peripheral B-cell pool is only slowly renewed after it has been initially built up early in life.
Similar articles
- Dividing cells in bone marrow and spleen incorporate bromodeoxyuridine with high efficiency.
Schittek B, Rajewsky K, Förster I. Schittek B, et al. Eur J Immunol. 1991 Jan;21(1):235-8. doi: 10.1002/eji.1830210136. Eur J Immunol. 1991. PMID: 1991489 - Analysis of B-cell life-span and homeostasis.
Förster I. Förster I. Methods Mol Biol. 2004;271:59-66. doi: 10.1385/1-59259-796-3:059. Methods Mol Biol. 2004. PMID: 15146112 - Peritoneal B cells regulate the numbers of allotype-matched pre-B and B cells in bone marrow.
Marcos MA, Sundblad A, Malenchère E, Coutinho A. Marcos MA, et al. Proc Natl Acad Sci U S A. 1991 Nov 15;88(22):9944-8. doi: 10.1073/pnas.88.22.9944. Proc Natl Acad Sci U S A. 1991. PMID: 1946463 Free PMC article. - B lymphocyte progenitors in mouse bone marrow.
Osmond DG, Park YH. Osmond DG, et al. Int Rev Immunol. 1987 Mar;2(3):241-61. doi: 10.3109/08830188709044756. Int Rev Immunol. 1987. PMID: 3333782 Review. No abstract available.
Cited by
- Rapid lymphocyte reconstitution of unconditioned immunodeficient mice with non-self-renewing multipotent hematopoietic progenitors.
Bhattacharya D, Bryder D, Rossi DJ, Weissman IL. Bhattacharya D, et al. Cell Cycle. 2006 Jun;5(11):1135-9. doi: 10.4161/cc.5.11.2772. Epub 2006 Jun 1. Cell Cycle. 2006. PMID: 16760650 Free PMC article. - Peripheral B cell selection and homeostasis.
Cancro MP, Smith SH. Cancro MP, et al. Immunol Res. 2003;27(2-3):141-8. doi: 10.1385/IR:27:2-3:141. Immunol Res. 2003. PMID: 12857963 Review. - Identification and characterization of circulating human transitional B cells.
Sims GP, Ettinger R, Shirota Y, Yarboro CH, Illei GG, Lipsky PE. Sims GP, et al. Blood. 2005 Jun 1;105(11):4390-8. doi: 10.1182/blood-2004-11-4284. Epub 2005 Feb 8. Blood. 2005. PMID: 15701725 Free PMC article. - B cell maturation and selection at the marrow-periphery interface.
Cancro MP, Allman DM, Hayes CE, Lentz VM, Fields RG, Sah AP, Tomayko M. Cancro MP, et al. Immunol Res. 1998;17(1-2):3-11. doi: 10.1007/BF02786425. Immunol Res. 1998. PMID: 9479562 Review. - B cell clonal elimination induced by membrane-bound self-antigen may require repeated antigen encounter or cell competition.
Lang J, Nemazee D. Lang J, et al. Eur J Immunol. 2000 Feb;30(2):689-96. doi: 10.1002/1521-4141(200002)30:2<689::AID-IMMU689>3.0.CO;2-I. Eur J Immunol. 2000. PMID: 10671228 Free PMC article.
References
- Cell Immunol. 1973 Apr;7(1):40-59 - PubMed
- Blood. 1965 Sep;26:281-95 - PubMed
- Cell Immunol. 1977 Sep;33(1):114-24 - PubMed
- Proc Natl Acad Sci U S A. 1978 Oct;75(10):4982-6 - PubMed
- Mol Immunol. 1979 Dec;16(12):1005-17 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical