The microaerophilic microbiota of de-novo paediatric inflammatory bowel disease: the BISCUIT study - PubMed (original) (raw)

doi: 10.1371/journal.pone.0058825. Epub 2013 Mar 12.

Susan H Berry, Indrani Mukhopadhya, John M Thomson, Karin A Saunders, Charlotte E Nicholl, W Michael Bisset, Sabarinathan Loganathan, Gamal Mahdi, Dagmar Kastner-Cole, Andy R Barclay, Jon Bishop, Diana M Flynn, Paraic McGrogan, Richard K Russell, Emad M El-Omar, Georgina L Hold

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The microaerophilic microbiota of de-novo paediatric inflammatory bowel disease: the BISCUIT study

Richard Hansen et al. PLoS One. 2013.

Abstract

Introduction: Children presenting for the first time with inflammatory bowel disease (IBD) offer a unique opportunity to study aetiological agents before the confounders of treatment. Microaerophilic bacteria can exploit the ecological niche of the intestinal epithelium; Helicobacter and Campylobacter are previously implicated in IBD pathogenesis. We set out to study these and other microaerophilic bacteria in de-novo paediatric IBD.

Patients and methods: 100 children undergoing colonoscopy were recruited including 44 treatment naïve de-novo IBD patients and 42 with normal colons. Colonic biopsies were subjected to microaerophilic culture with Gram-negative isolates then identified by sequencing. Biopsies were also PCR screened for the specific microaerophilic bacterial groups: Helicobacteraceae, Campylobacteraceae and Sutterella wadsworthensis.

Results: 129 Gram-negative microaerophilic bacterial isolates were identified from 10 genera. The most frequently cultured was S. wadsworthensis (32 distinct isolates). Unusual Campylobacter were isolated from 8 subjects (including 3 C. concisus, 1 C. curvus, 1 C. lari, 1 C. rectus, 3 C. showae). No Helicobacter were cultured. When comparing IBD vs. normal colon control by PCR the prevalence figures were not significantly different (Helicobacter 11% vs. 12%, p = 1.00; Campylobacter 75% vs. 76%, p = 1.00; S. wadsworthensis 82% vs. 71%, p = 0.312).

Conclusions: This study offers a comprehensive overview of the microaerophilic microbiota of the paediatric colon including at IBD onset. Campylobacter appear to be surprisingly common, are not more strongly associated with IBD and can be isolated from around 8% of paediatric colonic biopsies. S. wadsworthensis appears to be a common commensal. Helicobacter species are relatively rare in the paediatric colon.

Trial registration: This study is publically registered on the United Kingdom Clinical Research Network Portfolio (9633).

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Conflict of interest statement

Competing Interests: Georgina Hold is a PLOS ONE Editorial Board member. This does not alter the authors’ adherence to all the PLOS ONE policies on sharing data and materials.

Figures

Figure 1

Figure 1. Recruitment flowchart of recruits to BISCUIT study.

Those where recruitment was not possible were approached by post but could not then be recruited on their day of colonoscopy. The one child consented but not biopsied was due to unavailability of the investigator on the day in question.

Figure 2

Figure 2. Basic phenotypic assessment of 414 bacterial isolates obtained from the paediatric colonic mucosa.

129 were both Gram-negative and non-aerobic, of which 114 were formally identified by sequencing.

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