Structural basis for termination of AIM2-mediated signaling by p202 - PubMed (original) (raw)
Structural basis for termination of AIM2-mediated signaling by p202
Heng Ru et al. Cell Res. 2013 Jun.
No abstract available
Figures
Figure 1
Structural basis for the termination of AIM2-mediated signaling by p202. (A) Schematic diagram of domain arrangement of mAIM2 and p202. Arrows indicate the length of the construct used for structural studies. (B) Cartoon representation of the structure of the HIN domain of mAIM2 bound with DNA. (C) Side view of the cartoon representation of the structure of the HIN domain of mAIM2 bound with dsDNA (left panel). Right panel shows the top view of the structure showing a helix from the linker inserted into the DNA spiral. DNA strand are shown in blue and green. (D) Electrostatic surface potential representation of the HIN domain of mAIM2 bound with dsDNA. 2Fo-Fc electron density map for DNA contoured at 1σ is shown. Positive potential is shown in blue, negative potential in red. DNA is shown as sticks. (E) Cartoon representation of the structure of the HINa domain of p202. Secondary structural elements are labeled according to the canonical OB-fold convention. (F) Side view of the structure of p202-HINa bound with dsDNA (left panel). Right panel shows the top view of the same structure showing loops protruding out from the β-barrel of OB-fold. Strands of DNA are shown in blue and green. (G) Surface plasmon resonance (SPR) assay of the DNA-binding affinity of wild-type p202 HINa domain and its mutants harboring clusters of mutations (M3, M6, M9, M10, M13, M16 and M20) around the DNA-binding region. Data shown represent the binding to AT-rich dsDNA (5′-TTATATATATATATATATAA-3′). (H) Cartoon representation of the mAIM2 HIN molecules bound to dsDNA. (I) Modes of dsDNA binding by the HIN domains of p202 and AIM2. Cα atoms of the HINa domain of p202 (grey) bound with DNA (green ribbon) are superimposed over Cα atoms of the HIN domain of murine AIM2 (blue) bound with DNA (orange ribbon). (J) Cartoon of the p202 HINa molecules bound with DNA. (K) The HINa domain of p202 (green) binds more DNA than mAIM2 HIN domain (red) when assayed under identical conditions using equal molar ratios of protein and DNA.
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