The human TREX-2 complex is stably associated with the nuclear pore basket - PubMed (original) (raw)

. 2013 Jun 15;126(Pt 12):2656-67.

doi: 10.1242/jcs.118000. Epub 2013 Apr 16.

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The human TREX-2 complex is stably associated with the nuclear pore basket

David Umlauf et al. J Cell Sci. 2013.

Abstract

In eukaryotes, mRNA export involves many evolutionarily conserved factors that carry the nascent transcript to the nuclear pore complex (NPC). The THO/TREX complex couples transcription to mRNA export and recruits the mRNA export receptor NXF1 for the transport of messenger ribonucleoprotein particles (mRNP) to the NPC. The transcription and export complex 2 (TREX-2) was suggested to interact with NXF1 and to shuttle between transcription sites and the NPC. Here, we characterize the dynamics of human TREX-2 and show that it stably associates with the NPC basket. Moreover, the association of TREX-2 with the NPC requires the basket nucleoporins NUP153 and TPR, but is independent of transcription. Differential profiles of mRNA nuclear accumulation reveal that TREX-2 functions similarly to basket nucleoporins, but differently from NXF1. Thus, our results show that TREX-2 is an NPC-associated complex in mammalian cells and suggest that it is involved in putative NPC basket-related functions.

Keywords: AMEX; NXF1; Nuclear pore complex; THO/TREX; TREX-2; Transcription; mRNA export.

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