Insights into degron recognition by APC/C coactivators from the structure of an Acm1-Cdh1 complex - PubMed (original) (raw)

Insights into degron recognition by APC/C coactivators from the structure of an Acm1-Cdh1 complex

Jun He et al. Mol Cell. 2013.

Abstract

The anaphase-promoting complex/cyclosome (APC/C) regulates sister chromatid segregation and the exit from mitosis. Selection of most APC/C substrates is controlled by coactivator subunits (either Cdc20 or Cdh1) that interact with substrate destruction motifs--predominantly the destruction (D) box and KEN box degrons. How coactivators recognize D box degrons and how this is inhibited by APC/C regulatory proteins is not defined at the atomic level. Here, from the crystal structure of S. cerevisiae Cdh1 in complex with its specific inhibitor Acm1, which incorporates D and KEN box pseudosubstrate motifs, we describe the molecular basis for D box recognition. Additional interactions between Acm1 and Cdh1 identify a third protein-binding site on Cdh1 that is likely to confer coactivator-specific protein functions including substrate association. We provide a structural rationalization for D box and KEN box recognition by coactivators and demonstrate that many noncanonical APC/C degrons bind APC/C coactivators at the D box coreceptor.

Copyright © 2013 Elsevier Inc. All rights reserved.

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Figures

Figure 1

Figure 1

Structure of Acm1CIR-Cdh1WD40 (A) Schematic of S. cerevisiae Acm1. A, A motif; DB1, N-terminal D box; NEN, 90N-91E-92N; KEN and DB3, pseudosubstrate KEN and D box (DB3) inhibitory motifs; SP, S102 CDK2 phosphorylation site; P, pT161-14-3-3 binding site; K, K motif. (B) Acm1CIR-Cdh1WD40 heterotetramer. F113 of Acm1CIR-A was not located in the electron density maps and is represented with a dashed line. (C) Model for the Acm1CIR-Cdh1WD40 heterodimer. A 13 residue linker was modeled between P103 (KEN) and R119 (DB3) (separated by 15 residues), indicating that it is stereochemically possible for the KEN box and DB3 motif of Acm1 to interact with their respective sites on the same Cdh1 molecule. K123 of the DB3 was omitted for clarity. See also Figure S1.

Figure 2

Figure 2

The A Motif-Binding Site (A) Details of A motif-Cdh1WD40 interactions at the interblade groove. (B) Surface rendition of Cdh1WD40 at the A motif-binding site. (C) The modeled Acm1CIR-Cdh1WD40 heterodimer with the Cdh1WD40 surface representation colored according to the sequence conservation of Cdc20 and Cdh1. The A motif lies in an interblade channel, which is less well conserved than the D box- and KEN box-binding sites. (D) Comparison of Cdh1WD40 (green) and Cdc20WD40 (purple) at the A motif-binding site shows why Cdc20 is not inhibited by Acm1. The five residues of Cdh1 that contact the A motif and differ from Cdc20 (I311, L325, K333, I344, and P374) are shown. (E) Residues of Cdh1 that differ from Cdc20 (shown in C) were replaced with the Cdc20 equivalents, and WT and mutant Cdh1 proteins were tested for their sensitivity to Acm1-mediated inhibition. In vitro APC ubiquitination assays show that the mutant incorporating two key residues (Cdh1I331S/K333T) was poorly inhibited by Acm1 and a mutant incorporating all five residues (Cdh1I331S/K333T/L325N/I344G/P374K) was even less sensitive to Acm1-mediated inhibition.

Figure 3

Figure 3

The KEN Motif and NEN Sequence Bind to the Conserved KEN Box Site of Coactivators (A) Details of NEN sequence-Cdh1 interactions. (B) Comparison of the NEN sequence of Acm1CIR-B bound to Cdh1WD40-B and the KEN motif of S. pombe Mad3 bound to S. pombe Cdc20WD40. (C) Details of KEN box and CDK phosphorylation site (pS102)-Cdh1 interactions. (D) Comparison of the KEN motif of Acm1CIR-A bound to Cdh1WD40-A and the KEN motif of S. pombe Mad3 bound to S. pombe Cdc20WD40.

Figure 4

Figure 4

D Box Binds to an Interblade Groove in Cdh1 (A) Details of DB3 box-Cdh1 interactions showing the N-terminal seven residues of D box. (B) Surface rendition of Cdh1 at the D box-binding site. See also Figure S2.

Figure 5

Figure 5

Consensus D Box and KEN Box Motifs and Related Noncanonical APC/C Degrons (A) Sequence motif of D box derived from 68 APC/C substrates. Sequence motif determined using multiple expectation maximization for motif elicitation (MEME) (Bailey et al., 2009). The D box sequences used are listed in Figure S3A. (B) Sequence motif of KEN box derived from 46 APC/C substrates. KEN box sequences are listed in Figure S3B. (C) Comparison of consensus D box with O box degron (Araki et al., 2005), the S. cerevisiae Spo13 degron (Sullivan and Morgan, 2007), the S. cerevisiae Cin8p degron (Hildebrandt and Hoyt, 2001), the D box of human Sgo1 (Karamysheva et al., 2009), and DB3 of Acm1. Underlined residues are essential for APC/C-mediated degradation. Red: D box consensus R and L residues; blue: D box consensus residues; orange: consensus P−1 and P+1 residues. (D) Comparison of consensus KEN box with the GxEN degron of Xkid (Castro et al., 2003). Red: invariant residues; blue: conserved residues. (E) Structural rationale for conservation of D or N at P−1 of KEN motif. Side chain of D or N stabilizes the KEN box conformation through a hydrogen bond with the amide side chain of the KEN box N residue. Modeled on the KEN box of Mad3 (Chao et al., 2012).

Figure 6

Figure 6

Oligomeric States of Acm1-Cdh1WD40 (A) Size-exclusion chromatography of WT Acm1CIR-Cdh1WD40 without glycerol shows heterotetramers (peak 1) and heterodimers (peak 2). Glycerol (10% v/v) promotes heterotetramers. Associated SDS-PAGE gels are shown in Figures S4B and S4D. Only heterodimers are observed when either the NEN sequence (Acm1CIR-mNEN-Cdh1WD40) or DB3 is disrupted (Acm1CIR-mdb3-Cdh1WD40). The latter two samples were run with 10% (v/v) glycerol. Associated SDS-PAGE gels shown in Figures S4I and S4J. (B and C) SAXS profiles for Acm1CIR-Cdh1WD40 and Acm1CIR-mNEN-Cdh1WD40 complexes. (B) Experimental SAXS profile (black dots) for Acm1CIR-Cdh1WD40 compared with computed SAXS profiles for the Acm1CIR-Cdh1WD40 tetramer (cyan) and dimer (red). It shows a good fit to the computed SAXS profile of the tetramer (χ2 = 12) but is a poor match to the computed SAXS profile of the dimer (χ2 = 93). The experimental radius of gyration (Rg) for Acm1CIR-Cdh1WD40 is 31 Å, and the calculated radii of gyration for tetramer and dimer models are 28 Å and 20 Å, respectively. The scattering vector q = 4πsin(θ)/λ, where θ is half the scattering angle. (C) Experimental SAXS profile (black dots) of Acm1CIR-mNEN-Cdh1WD40 shows a good fit to the computed SAXS profile of the dimer (χ2 = 3.7) but is a poor match to the computed SAXS profile of the tetramer (χ2 = 9.6). Experimental radius of gyration is 25 Å. (D) In vitro APC ubiquitination assays with 35S-labeled S. cerevisiae securin and IVT-produced _Sc_Cdh1 and WT and mutant Acm1CIR. Acm1CIR-mediated inhibition is abolished by mutation of the DB3 (Acm1CIR-mdb3). In contrast to DB3 mutations, disruption of the NEN sequence (Acm1CIR-mNEN) does not impair Acm1CIR-mediated inhibition of APC/CCdh1 ubiquitination of securin.

Figure 7

Figure 7

Noncanonical D and KEN Boxes Interact with D and KEN Box Recognition Sites (A) Ubiquitination of S. cerevisiae securin, S.cerevisiae Spo13, S. cerevisiae Cin8p, and X. laevis Xkid relies on recognition of APC/CCdh1 by the D box coreceptor. Ubiquitination is inhibited by a D box peptide (D) but not by a control peptide (C). (B) A conventional D box and noncanonical D box peptides (at 1 mM) reduce ubiquitination of 35S-labeled S. cerevisiae Clb2 with a disrupted KEN box (Clb2 mkb) to a level similar to that of Clb2 mdk with disrupted D and KEN boxes in the absence of peptide. (C) Xkid ubiquitination is dependent on the KEN box recognition site of Cdh1. Ubiquitination is abolished by disrupting either the D box receptor (mdr: _Hs_Cdh1V203M) or KEN box receptor (mkr: _Hs_Cdh1Q401A/R445L) of Cdh1. WT: wild-type _Hs_Cdh1. Human securin is used as a control.

Comment in

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