Generation of guanine-thymine cross-links in human cells by one-electron oxidation mechanisms - PubMed (original) (raw)

. 2013 Jul 15;26(7):1031-3.

doi: 10.1021/tx400158g. Epub 2013 Jun 11.

Affiliations

• PMID: 23734842
• PMCID: PMC3812307
• DOI: 10.1021/tx400158g

Generation of guanine-thymine cross-links in human cells by one-electron oxidation mechanisms

Guru S Madugundu et al. Chem Res Toxicol. 2013.

Abstract

The one-electron oxidation of cellular DNA in cultured human HeLa cells initiated by intense nanosecond 266 nm laser pulse irradiation produces cross-links between guanine and thymine bases (G*-T*), characterized by a covalent bond between C8 guanine (G*) and N3 thymine (T*) atoms. The DNA lesions were quantified by isotope dilution LC-MS/MS methods in the multiple reaction-monitoring mode using isotopically labeled [(15)N, (13)C]-nucleotides as internal standards. Among several known pyrimidine and 8-oxo-7,8-dihydroguanine lesions, the G*-T* cross-linked lesions were detected at levels of ~0.21 and 1.19 d(G*-T*) lesions per 10(6) DNA bases at laser intensities of 50 and 280 mJ/cm(2)/pulse, respectively.

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Conflict of interest statement

The authors declare no competing financial interest.

Figures

Chart 1

Structures of DNA lesions.

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