TNF-α triggers osteogenic differentiation of human dental pulp stem cells via the NF-κB signalling pathway - PubMed (original) (raw)

. 2013 Dec;37(12):1267-75.

doi: 10.1002/cbin.10141. Epub 2013 Oct 21.

Guijuan Feng, Jing Xing, Biyu Shen, Liren Li, Wei Tan, Yue Xu, Suzhe Liu, Hong Liu, Jinxia Jiang, Hao Wu, Tao Tao, Zhifeng Gu

Affiliations

TNF-α triggers osteogenic differentiation of human dental pulp stem cells via the NF-κB signalling pathway

Xingmei Feng et al. Cell Biol Int. 2013 Dec.

Abstract

Dental pulp stem cells (DPSCs) are a type of mesenchymal stem cells (MSCs) characterised by self-renewal and multi-lineage differentiation, including chondrocytes, adipocytes, neural cells and osteoblasts, which make it an attractive choice for tissue engineering purposes. Tumour necrosis factor α (TNF-α) had the positive effect on the mineralisation of bone marrow MSCs and stromal cells derived from human adipose tissue. However, the effect of TNF-α on DPSCs is unclear. We found that TNF-α activated the NF-κB pathway during the osteogenic differentiation of DPSCs. TNF-α also increased mineralisation and the expression of bone morphogenetic protein 2 (BMP2), alkaline phosphatase (ALP), runt-related transcription factor 2 (RUNX2) and collagen type I (COL I) during this process. PDTC, an NF-κB inhibitor, blocked the osteogenic differentiation induced by TNF-α. No effect of TNF-α on proliferation of DPSCs or cell cycle was detected. In summary, TNF-α promotes mineralisation and mineralisation-related gene expression through the NF-κB signalling pathway in DPSCs, which may provide a foundation for autologous transplantation of DPSCs.

Keywords: dental pulp stem cells (DPSCs); nuclear factor kappaB (NF-κB); osteogenic differentiation; tumour necrosis factor α (TNF-α).

© 2013 International Federation for Cell Biology.

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