Piceatannol enhances cisplatin sensitivity in ovarian cancer via modulation of p53, X-linked inhibitor of apoptosis protein (XIAP), and mitochondrial fission - PubMed (original) (raw)

Effects of piceatannol on CDDP treatment in OVCA cell lines. A, reductions in cell viability caused by PCT (10 μ

m

) in combination with CDDP treatment (10 μ

m

, 24 h) in chemosensitive and chemoresistant OVCA. Piceatannol (10 μ

m

) enhances the effects of CDDP (10 μ

m

, 24 h) in OV2008, A2780s, and C13 cells. Although piceatannol did not confer any additional effects on CDDP treatment in p53-null SKOV-3, it enhanced the effects of CDDP (10 μ

m

) in OVCAR-432, a chemosensitive p53-mutant cell line. B, time course study on the effects of PCT (10 μ

m

) and CDDP (10 μ

m

), alone and together, on cell viability of OV2008 cells over 48 h. C, effects of CDDP (5 μ

m

), piceatannol (10 μ

m

), and combined treatment on nuclear morphology in OV2008 cells after 24 h (stained with Hoechst 33258). Dual treatment significantly increases the severity of nuclear condensation and fragmentation in comparison to either agent alone. D, piceatannol treatment (2.5 μ

m

, 24 h) induces a left shift in concentration-response curves for CDDP-induced apoptosis in chemosensitive OV2008 and A2780s. Both cell lines also exhibited a left shift in apoptotic response curves to piceatannol when in the presence of low CDDP concentration (5 μ

m

). E, piceatannol induces a left shift in concentration-response curves for CDDP-induced apoptosis in chemoresistant C13* cells and induces sensitivity in combination with CDDP treatment (10 μ

m

, 24 h) in chemoresistant p53-mutant A2780cp* at 10 μ

m

concentration. *, p < 0.05; **, p < 0.01; ***, p < 0.001 versus respective dimethyl sulfoxide (DMSO) control (CTL).