Molecular cloning and functional expression of a potassium channel cDNA isolated from a rat cardiac library - PubMed (original) (raw)

Molecular cloning and functional expression of a potassium channel cDNA isolated from a rat cardiac library

J C Tseng-Crank et al. FEBS Lett. 1990.

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Abstract

A full-length K+ channel cDNA (RHK1) was isolated from a rat cardiac library using the polymerase chain reaction (PCR) method and degenerate oligonucleotide primers derived from K+ channel sequences conserved between Drosophila Shaker H4 and mouse brain MBK1. Although RHK1 was isolated from heart, its expression was found in both heart and brain. The RHK1-encoded protein, when expressed in Xenopus oocytes, gated a 4-aminopyridine (4-AP)-sensitive transient outward current. This current is similar to the transient outward current measured in rat ventricular myocytes with respect to voltage-dependence of activation and inactivation, time course of activation and inactivation, and pharmacology.

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