Interrogating the relationship between naïve and immune antiviral T cell repertoires - PubMed (original) (raw)

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Interrogating the relationship between naïve and immune antiviral T cell repertoires

Nicole L La Gruta et al. Curr Opin Virol. 2013 Aug.

Abstract

Understanding how naïve virus-specific CD8+ T cells influence the type of immune response generated after virus infection is critical for the development of enhanced therapeutic and vaccination strategies to exploit CD8+ T cell-mediated immunity. Recent technological advances in T cell isolation and T receptor sequencing have allowed for greater understanding of the basic structure of immune T cell repertoires, the diversity of responses within and between individuals, and changes in repertoires over time and in response to infection conditions. In this review, we discuss the current understanding of how T cell repertoires contribute to potent antiviral responses. Additionally we compare the state of the art in receptor sequencing, highlighting the advantages and disadvantages of the three most common approaches: next-generation sequencing, template-switch anchored RT-PCR, and multiplex single cell PCR. Finally, we describe how TCR sequencing has delineated the relationship between naïve and immune T cell repertoires.

Copyright © 2013 Elsevier B.V. All rights reserved.

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Figures

Figure 1

Figure 1. Influence of the naïve T cell repertoire on the antiviral immune response

A schematic depicting the clonotypes present in the naïve and antiviral immune repertoires of three mice. Each letter corresponds to a unique clonotype. The antiviral immune repertoire broadly reflects the characteristics of the naïve repertoire, but for all epitopes shows significantly greater inequality in the distribution of clonotypes relative to the naïve repertoire, with some responses expand to a greater extent than others (e.g. clonotypes C, G, J and O). The extent of sharing is largely determined in the naïve repertoire (e.g. clonotypes A and B) and reflects sharing of both minor and dominant clones.

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