Reprogramming of human fibroblasts to pluripotency with lineage specifiers - PubMed (original) (raw)
. 2013 Sep 5;13(3):341-50.
doi: 10.1016/j.stem.2013.06.019. Epub 2013 Jul 18.
Affiliations
- PMID: 23871606
- DOI: 10.1016/j.stem.2013.06.019
Free article
Reprogramming of human fibroblasts to pluripotency with lineage specifiers
Nuria Montserrat et al. Cell Stem Cell. 2013.
Free article
Abstract
Since the initial discovery that OCT4, SOX2, KLF4, and c-MYC overexpression sufficed for the induction of pluripotency in somatic cells, methodologies replacing the original factors have enhanced our understanding of the reprogramming process. However, unlike in mouse, OCT4 has not been replaced successfully during reprogramming of human cells. Here we report on a strategy to accomplish this replacement. Through a combination of transcriptome and bioinformatic analysis we have identified factors previously characterized as being lineage specifiers that are able to replace OCT4 and SOX2 in the reprogramming of human fibroblasts. Our results show that it is possible to replace OCT4 and SOX2 simultaneously with alternative lineage specifiers in the reprogramming of human cells. At a broader level, they also support a model in which counteracting lineage specification networks underlies the induction of pluripotency.
Copyright © 2013 Elsevier Inc. All rights reserved.
Comment in
- And then there were none: no need for pluripotency factors to induce reprogramming.
Chou BK, Cheng L. Chou BK, et al. Cell Stem Cell. 2013 Sep 5;13(3):261-2. doi: 10.1016/j.stem.2013.08.004. Cell Stem Cell. 2013. PMID: 24012365 Free PMC article.
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