Novel cyclovirus in human cerebrospinal fluid, Malawi, 2010-2011 - PubMed (original) (raw)

Novel cyclovirus in human cerebrospinal fluid, Malawi, 2010-2011

Saskia L Smits et al. Emerg Infect Dis. 2013.

Abstract

To identify unknown human viruses, we analyzed serum and cerebrospinal fluid samples from patients with unexplained paraplegia from Malawi by using viral metagenomics. A novel cyclovirus species was identified and subsequently found in 15% and 10% of serum and cerebrospinal fluid samples, respectively. These data expand our knowledge of cyclovirus diversity and tropism.

Keywords: Malawi; Paraplegia; cerebrospinal fluid; cyclovirus; random amplification; serum; virus; viruses.

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Figures

Figure

Figure

Cyclovirus genome organization and phylogenetic analysis of translated putative replication-associated protein (Rep) sequences. A) Size and predicted genome organization of human cyclovirus VS5700009, showing the 2 major open reading frames (ORFs) encoding Rep and the putative capsid protein (Cap, and other ORFs with a coding capacity >100 aa (ORFs 3–5). B) Phylogenetic tree of the translated Rep sequence of human cyclovirus VS5700009 and representative human and animal cycloviruses, generated by using MEGA5 with the neighbor-joining method with p-distance and 1,000 bootstrap replicates. A chimpanzee circovirus was used as an outgroup. Significant bootstrap values are shown. Cycloviruses in the same species are defined as having >85% aa identity in the Rep region and are labeled by vertical bars as described (11). The human (white), dragonfly (blue), bat (red), goat (pink), cattle (black), chicken (green), and chimpanzee (gray) cycloviruses are indicated, and VS5700009 is highlighted in red. Scale bar = 5% estimated phylogenetic divergence. C) Phylogenetic tree of the genomic area, corresponding to nt 392–733 in human cyclovirus VS5700009 and representative human and animal cycloviruses, generated by using MEGA5 with the neighbor-joining method with p-distance and 1,000 bootstrap replicates. Significant bootstrap values are shown. Cycloviruses isolated from the same species are labeled by vertical bars as described (11). Cycloviruses from humans (white), cattle (black), and chimpanzees (gray) are indicated, and VS5700009 is highlighted in red. Scale bar = 10% estimated phylogenetic divergence.

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