DMSO enhances electrospray response, boosting sensitivity of proteomic experiments - PubMed (original) (raw)
. 2013 Oct;10(10):989-91.
doi: 10.1038/nmeth.2610. Epub 2013 Aug 25.
Affiliations
- PMID: 23975139
- DOI: 10.1038/nmeth.2610
DMSO enhances electrospray response, boosting sensitivity of proteomic experiments
Hannes Hahne et al. Nat Methods. 2013 Oct.
Abstract
We report that low percentages of dimethylsulfoxide (DMSO) in liquid chromatography solvents lead to a strong enhancement of electrospray ionization of peptides, improving the sensitivity of protein identification in bottom-up proteomics by up to tenfold. The method can be easily implemented on any LC-MS/MS system without modification to hardware or software and at no additional cost.
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