Assembly, remodelled - PubMed (original) (raw)

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Assembly, remodelled

Karim Bouazoune et al. Elife. 2013.

Abstract

Biochemical assays reveal that nucleosome maturation and chromatin remodelling by the motor protein Chd1 are distinct, separable enzymatic activities.

Keywords: Chd1; D. melanogaster; S. cerevisiae; chromatin; chromatin assembly; chromatin remodelling; motor protein; nucleosome.

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Conflict of interest statement

Competing interests:The authors declare that no competing interests exist.

Figures

Figure 1.. Mature nucleosome arrays are formed in several stages.

Histone proteins are first loaded onto DNA to create non-canonical nucleosomal structures referred to as ‘prenucleosomes’ (top and second panels). The core histones, H2A, H2B, H3 and H4, associate with each other in heterodimers (H2A-H2B and H3-H4; top panel) and are deposited onto DNA in a specific order by proteins called histone chaperones (top blue box) to form an irregularly spaced prenucleosome array (dashed ovals, second panel). Mature nucleosomes are then formed, and also repositioned to ensure their even spacing on the DNA, by ATP-dependent chromatin remodelling factors (motor proteins; lower blue box). Maturation and repositioning were formerly thought to be achieved by a single enzymatic function (long arrow at left, bottom panel). Now, using mutant variants of a motor protein called Chd1, Torigoe et al. suggest that chromatin remodelling factors use not one but two distinct ATP-dependent activities to convert randomly deposited prenucleosomes into a mature array of evenly spaced nucleosomes (solid ovals; short arrows at right, third and bottom panels).

Comment on

ATP-dependent chromatin assembly is functionally distinct from chromatin remodeling.
Torigoe SE, Patel A, Khuong MT, Bowman GD, Kadonaga JT. Torigoe SE, et al. Elife. 2013 Aug 20;2:e00863. doi: 10.7554/eLife.00863. Elife. 2013. PMID: 23986862 Free PMC article.

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The prenucleosome, a stable conformational isomer of the nucleosome.
Fei J, Torigoe SE, Brown CR, Khuong MT, Kassavetis GA, Boeger H, Kadonaga JT. Fei J, et al. Genes Dev. 2015 Dec 15;29(24):2563-75. doi: 10.1101/gad.272633.115. Genes Dev. 2015. PMID: 26680301 Free PMC article.

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Assembly, remodelled - PubMed (original) (raw)