Ordered splicing of thymidine kinase pre-mRNA during the S phase of the cell cycle - PubMed (original) (raw)
Ordered splicing of thymidine kinase pre-mRNA during the S phase of the cell cycle
J M Gudas et al. Mol Cell Biol. 1990 Oct.
Abstract
Concomitant with the onset of S phase, a series of thymidine kinase (TK) splicing intermediates as well as mature TK mRNA accumulates in the nucleus of BALB/c 3T3 cells. Most of the TK splicing intermediates are retained by oligo(dT)-cellulose chromatography, and, therefore, 3' end formation and polyadenylation probably precede the splicing of TK pre-mRNAs. We have further characterized the TK pre-mRNAs that are present in the nuclei of S-phase cells by using specific probes derived from each of the six TK intervening sequences. Based on the sizes of the pre-mRNAs and their patterns of hybridization with these intron probes, we propose a pathway for intron removal from nascent TK transcripts. Intron excision occurred by a preferred, but not necessarily obligatory, order which appears to have been conserved in mouse and Chinese hamster cells.
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References
- Cell. 1984 Dec;39(3 Pt 2):589-602 - PubMed
- Mol Cell Biol. 1984 Sep;4(9):1777-84 - PubMed
- Proc Natl Acad Sci U S A. 1985 Jul;82(13):4346-50 - PubMed
- Annu Rev Biochem. 1986;55:1119-50 - PubMed
- Mol Cell Biol. 1985 Nov;5(11):3149-56 - PubMed
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