TRAP assists membrane protein topogenesis at the mammalian ER membrane - PubMed (original) (raw)
. 2013 Dec;1833(12):3104-3111.
doi: 10.1016/j.bbamcr.2013.08.018. Epub 2013 Sep 5.
Affiliations
- PMID: 24013069
- DOI: 10.1016/j.bbamcr.2013.08.018
Free article
TRAP assists membrane protein topogenesis at the mammalian ER membrane
Nicole Sommer et al. Biochim Biophys Acta. 2013 Dec.
Free article
Abstract
Membrane protein insertion and topogenesis generally occur at the Sec61 translocon in the endoplasmic reticulum membrane. During this process, membrane spanning segments may adopt two distinct orientations with either their N- or C-terminus translocated into the ER lumen. While different topogenic determinants in membrane proteins, such as flanking charges, polypeptide folding, and hydrophobicity, have been identified, it is not well understood how the translocon and/or associated components decode them. Here we present evidence that the translocon-associated protein (TRAP) complex is involved in membrane protein topogenesis in vivo. Small interfering RNA (siRNA)-mediated silencing of the TRAP complex in HeLa cells enhanced the topology effect of mutating the flanking charges of a signal-anchor, but not of increasing signal hydrophobicity. The results suggest a role of the TRAP complex in moderating the 'positive-inside' rule.
Keywords: Endoplasmic reticulum; Membrane protein topogenesis; RNA interference; Sec61 complex; Translocon associated proteins.
© 2013.
Similar articles
- Dissecting the molecular organization of the translocon-associated protein complex.
Pfeffer S, Dudek J, Schaffer M, Ng BG, Albert S, Plitzko JM, Baumeister W, Zimmermann R, Freeze HH, Engel BD, Förster F. Pfeffer S, et al. Nat Commun. 2017 Feb 20;8:14516. doi: 10.1038/ncomms14516. Nat Commun. 2017. PMID: 28218252 Free PMC article. - The expanding role of the ER translocon in membrane protein folding.
Skach WR. Skach WR. J Cell Biol. 2007 Dec 31;179(7):1333-5. doi: 10.1083/jcb.200711107. J Cell Biol. 2007. PMID: 18166647 Free PMC article. Review. - Proteomics reveals signal peptide features determining the client specificity in human TRAP-dependent ER protein import.
Nguyen D, Stutz R, Schorr S, Lang S, Pfeffer S, Freeze HH, Förster F, Helms V, Dudek J, Zimmermann R. Nguyen D, et al. Nat Commun. 2018 Sep 14;9(1):3765. doi: 10.1038/s41467-018-06188-z. Nat Commun. 2018. PMID: 30217974 Free PMC article. - Membrane insertion and topology of the translocon-associated protein (TRAP) gamma subunit.
Bañó-Polo M, Martínez-Garay CA, Grau B, Martínez-Gil L, Mingarro I. Bañó-Polo M, et al. Biochim Biophys Acta Biomembr. 2017 May;1859(5):903-909. doi: 10.1016/j.bbamem.2017.01.027. Epub 2017 Jan 26. Biochim Biophys Acta Biomembr. 2017. PMID: 28132902 - Topogenesis of membrane proteins at the endoplasmic reticulum.
Higy M, Junne T, Spiess M. Higy M, et al. Biochemistry. 2004 Oct 12;43(40):12716-22. doi: 10.1021/bi048368m. Biochemistry. 2004. PMID: 15461443 Review.
Cited by
- Molecular view of ER membrane remodeling by the Sec61/TRAP translocon.
Karki S, Javanainen M, Rehan S, Tranter D, Kellosalo J, Huiskonen JT, Happonen L, Paavilainen V. Karki S, et al. EMBO Rep. 2023 Dec 6;24(12):e57910. doi: 10.15252/embr.202357910. Epub 2023 Nov 20. EMBO Rep. 2023. PMID: 37983950 Free PMC article. - Expanding the Molecular and Clinical Phenotype of SSR4-CDG.
Ng BG, Raymond K, Kircher M, Buckingham KJ, Wood T, Shendure J, Nickerson DA, Bamshad MJ; University of Washington Center for Mendelian Genomics; Wong JT, Monteiro FP, Graham BH, Jackson S, Sparkes R, Scheuerle AE, Cathey S, Kok F, Gibson JB, Freeze HH. Ng BG, et al. Hum Mutat. 2015 Nov;36(11):1048-51. doi: 10.1002/humu.22856. Epub 2015 Aug 27. Hum Mutat. 2015. PMID: 26264460 Free PMC article. - Protein biosynthesis at the ER: finding the right accessories.
Shao S. Shao S. Mol Biol Cell. 2023 Jan 1;34(1):pe1. doi: 10.1091/mbc.E21-09-0451. Mol Biol Cell. 2023. PMID: 36520029 Free PMC article. - Understanding the mammalian TRAP complex function(s).
Russo A. Russo A. Open Biol. 2020 May;10(5):190244. doi: 10.1098/rsob.190244. Epub 2020 May 27. Open Biol. 2020. PMID: 32453970 Free PMC article. Review. - Components and Mechanisms of Import, Modification, Folding, and Assembly of Immunoglobulins in the Endoplasmic Reticulum.
Zimmermann R. Zimmermann R. J Clin Immunol. 2016 May;36 Suppl 1:5-11. doi: 10.1007/s10875-016-0250-0. Epub 2016 Feb 29. J Clin Immunol. 2016. PMID: 26923573 Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources