Murine models of human wound healing - PubMed (original) (raw)

Murine models of human wound healing

Jerry S Chen et al. Methods Mol Biol. 2013.

Abstract

In vivo wound healing experiments remain the most predictive models for studying human wound healing, allowing an accurate representation of the complete wound healing environment including various cell types, environmental cues, and paracrine interactions. Small animals are economical, easy to maintain, and allow researchers to take advantage of the numerous transgenic strains that have been developed to investigate the specific mechanisms involved in wound healing and regeneration. Here we describe three reproducible murine wound healing models that recapitulate the human wound healing process.

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Figures

Fig. 1

Fig. 1

Splinted excisional wound model. (a) The 16 mm punch is used to cut out the desired number of splints. (b) The 10 mm punch is then placed inside each silicone circle to remove the inner diameter and create the silicone splint. (c) A 6 mm punch is used to mark the location of the excisional wound. (d) Forceps retract the skin outward and scissors are used to sharply excise a circular piece of skin down through the panniculus carnosus. (e) The silicone splint is glued concentrically around the excisional wound. (f) Eight interrupted stitches reinforce the splint to the intact tissue surrounding the wound to prevent wound edge contracture

Fig. 2

Fig. 2

Ischemia reperfusion model. (a) Use a template to mark the position of the magnets half way between the fore and hindlimbs. Note a 1 cm skin bridge between the magnet placement. (b) Pinch fold of skin and place magnets around skin fold. (c) Representative animal on day 5 displaying bilateral stage 3 ulcers

Fig. 3

Fig. 3

Ischemic flap model. (a) Mark a 10 mm × 25 mm rectangle with the long dimension in the cranial–caudal axis. The cranial edge of the rectangle should be between the scapulae. (b) Raise a full-thickness flap leaving the cranial pedicle intact. (c) Place the impermeable silicone sheet flat on the wound and lay the flap down over the sheet. Note the superficial perforators that can be seen originating from the pedicle. (d) Suture the flap down using interrupted stitches. (e) Schematic of the O2 gradient produced with the ischemic flap model

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