Effects of pH, glucose, and chelating agents on lethality of paraquat to Escherichia coli - PubMed (original) (raw)
Effects of pH, glucose, and chelating agents on lethality of paraquat to Escherichia coli
H Minakami et al. J Bacteriol. 1990 Feb.
Abstract
Retention of paraquat by Escherichia coli B was greatest after exposure at pH 9.0 and was progressively less after exposure at pH 7.0 and 5.0, respectively. This retained paraquat was capable of persistent growth inhibition. Uptake and retention of paraquat by E. coli B was dependent upon a carbon source, such as glucose. Under comparable conditions E. coli K-12 did not retain paraquat. The lethality of paraquat was seen in TSY medium but not in VB medium. The addition of Soytone, tryptone, or yeast extract, to the VB medium allowed the lethality of paraquat to be seen. A variety of chelating agents, including EDTA, 8-hydroxyquinoline, and o-phenanthroline, prevented the lethal effect of paraquat in TSY medium. Although EDTA protected against the lethality of paraquat, it did not protect against its bacteriostatic effect.
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References
- Biochim Biophys Acta. 1981 Dec;650(2-3):151-66 - PubMed
- J Biol Chem. 1985 Sep 5;260(19):10478-81 - PubMed
- J Bacteriol. 1986 Jul;167(1):346-9 - PubMed
- J Biol Chem. 1986 Sep 25;261(27):12472-6 - PubMed
- Mutat Res. 1986 Oct;163(1):15-22 - PubMed
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