Translation initiation and ribosomal biogenesis: involvement of a putative rRNA helicase and RPL46 - PubMed (original) (raw)

. 1990 Mar 2;247(4946):1077-9.

doi: 10.1126/science.2408148.

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Translation initiation and ribosomal biogenesis: involvement of a putative rRNA helicase and RPL46

A B Sachs et al. Science. 1990.

Abstract

Cold-sensitive mutations in the SPB genes (spb1-spb7) of Saccharomyces cerevisiae suppress the inhibition of translation initiation resulting from deletion of the poly(A)-binding protein gene (PAB1). The SPB4 protein belongs to a family of adenosine triphosphate (ATP)-dependent RNA helicases. The aberrant production of 25S ribosomal RNA (rRNA) occurring in spb4-1 mutants or the deletion of SPB2 (RPL46) permits the deletion of PAB1. These data suggest that mutations affecting different steps of 60S subunit formation can allow PAB-independent translation, and they indicate that further characterization of the spb mutations could lend insight into the biogenesis of the ribosome.

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