Separation and partial characterization of three functional steps in transcription initiation by human RNA polymerase II - PubMed (original) (raw)
. 1985 Jul 5;260(13):8163-72.
- PMID: 2409080
Free article
Separation and partial characterization of three functional steps in transcription initiation by human RNA polymerase II
D K Hawley et al. J Biol Chem. 1985.
Free article
Abstract
We have used Sarkosyl to study the events comprising specific transcription initiation in vitro by HeLa RNA polymerase II. On the basis of different sensitivities to the Sarkosyl concentration, we have defined three functional steps in initiation at the adenovirus major late promoter: 1. a template commitment step that occurs in the presence of 0.015% Sarkosyl; 2. a nucleotide-independent conversion of the committed complex to a "rapid start complex" capable of initiating an RNA chain, a step blocked by 0.015% Sarkosyl; and 3. a step that requires nucleoside triphosphates, converts the rapid start complex to a stably initiated complex, and is sensitive to Sarkosyl concentrations greater than 0.05%. The subsequent elongation of the initiated RNA chain is resistant to Sarkosyl, except that Sarkosyl causes pausing or premature termination at a specific site about 186 nucleotides downstream of the major late cap site. Using this assay, we have further characterized these steps and the resulting intermediate complexes.
Similar articles
- Analysis of Tat transactivation of human immunodeficiency virus transcription in vitro.
Bohan CA, Kashanchi F, Ensoli B, Buonaguro L, Boris-Lawrie KA, Brady JN. Bohan CA, et al. Gene Expr. 1992;2(4):391-407. Gene Expr. 1992. PMID: 1282057 Free PMC article. - Promoter-dependent transcription by RNA polymerase II using immobilized enzyme complexes.
Arias JA, Dynan WS. Arias JA, et al. J Biol Chem. 1989 Feb 25;264(6):3223-9. J Biol Chem. 1989. PMID: 2464595 - Promoter escape by RNA polymerase II.
Dvir A. Dvir A. Biochim Biophys Acta. 2002 Sep 13;1577(2):208-223. doi: 10.1016/s0167-4781(02)00453-0. Biochim Biophys Acta. 2002. PMID: 12213653 Review.
Cited by
- PRO-seq: Precise Mapping of Engaged RNA Pol II at Single-Nucleotide Resolution.
Mimoso CA, Goldman SR. Mimoso CA, et al. Curr Protoc. 2023 Dec;3(12):e961. doi: 10.1002/cpz1.961. Curr Protoc. 2023. PMID: 38149731 - TFIID Enables RNA Polymerase II Promoter-Proximal Pausing.
Fant CB, Levandowski CB, Gupta K, Maas ZL, Moir J, Rubin JD, Sawyer A, Esbin MN, Rimel JK, Luyties O, Marr MT, Berger I, Dowell RD, Taatjes DJ. Fant CB, et al. Mol Cell. 2020 May 21;78(4):785-793.e8. doi: 10.1016/j.molcel.2020.03.008. Epub 2020 Mar 30. Mol Cell. 2020. PMID: 32229306 Free PMC article. - Isolation and genome-wide characterization of cellular DNA:RNA triplex structures.
Sentürk Cetin N, Kuo CC, Ribarska T, Li R, Costa IG, Grummt I. Sentürk Cetin N, et al. Nucleic Acids Res. 2019 Mar 18;47(5):2306-2321. doi: 10.1093/nar/gky1305. Nucleic Acids Res. 2019. PMID: 30605520 Free PMC article. - SRC-3 Coactivator Governs Dynamic Estrogen-Induced Chromatin Looping Interactions during Transcription.
Panigrahi AK, Foulds CE, Lanz RB, Hamilton RA, Yi P, Lonard DM, Tsai MJ, Tsai SY, O'Malley BW. Panigrahi AK, et al. Mol Cell. 2018 May 17;70(4):679-694.e7. doi: 10.1016/j.molcel.2018.04.014. Mol Cell. 2018. PMID: 29775582 Free PMC article. - Nascent RNA sequencing reveals distinct features in plant transcription.
Hetzel J, Duttke SH, Benner C, Chory J. Hetzel J, et al. Proc Natl Acad Sci U S A. 2016 Oct 25;113(43):12316-12321. doi: 10.1073/pnas.1603217113. Epub 2016 Oct 11. Proc Natl Acad Sci U S A. 2016. PMID: 27729530 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Miscellaneous