Time-resolved fluorescence anisotropy imaging - PubMed (original) (raw)
Time-resolved fluorescence anisotropy imaging
Klaus Suhling et al. Methods Mol Biol. 2014.
Abstract
Fluorescence can be characterized by its intensity, position, wavelength, lifetime, and polarization. The more of these features are acquired in a single measurement, the more can be learned about the sample, i.e., the microenvironment of the fluorescence probe. Polarization-resolved fluorescence lifetime imaging-time-resolved fluorescence anisotropy imaging, TR-FAIM-allows mapping of viscosity or binding or of homo-FRET which can indicate dimerization or generally oligomerization.
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